Department of General Surgery, The First Affiliated Hospital, Sun Yat-sen University, No. 58 Zhongshan 2nd Road, 510080 Guangzhou, China P.R.
Department of Oral and Maxillofacial Surgery, Shanghai Ninth People-s Hospital affiliated to Shanghai Jiao Tong University School of Medicine, 639 Zhizaoju Road, Huangpu, 200011 Shanghai, China P.R.
Benef Microbes. 2015 Mar;6(1):113-8. doi: 10.3920/BM2013.0095.
Intestinal mucositis is a common toxic side effect in cancer patients receiving high-dose chemotherapy. This study aimed to evaluate the beneficial effects of Bifidobacterium infantis in a rat model of intestinal mucositis induced by 5-fluorouracil (5-FU). Thirty male Sprague-Dawley rats were divided into three groups: control, 5-FU, and 5-FU + B. infantis. A single intraperitoneal injection of 5-FU (150 mg/kg) was used to induce intestinal mucositis. B. infantis (1×109 cfu) was administered for 11 days, starting from 7 days before 5-FU injection. Intestinal mucositis was evaluated based on body weight, villus height, immunohistological expression of proliferating cell nuclear antigen (PCNA) and nuclear factor kappa beta (NF-κB), levels of the pro-inflammatory factors interleukin 1 beta and tumour necrosis factor alpha, and myeloperoxidase (MPO) concentration. The results showed that the 5-FU + B. infantis group demonstrated a higher body weight and villus height, increased expression of PCNA, reduced expression of NF-κB and pro-inflammatory factors, and lower MPO concentration compared to the 5-FU group. These data suggest that probiotic B. infantis is effective in reducing chemotherapy-induced intestinal mucositis in rats.
肠黏膜炎是癌症患者接受大剂量化疗时常见的毒性副作用。本研究旨在评估婴儿双歧杆菌在氟尿嘧啶（5-FU）诱导的大鼠肠黏膜炎模型中的有益作用。将 30 只雄性 Sprague-Dawley 大鼠分为三组：对照组、5-FU 组和 5-FU+B. infantis 组。通过单次腹腔注射 5-FU（150mg/kg）诱导肠黏膜炎。B. infantis（1×109cfu）于 5-FU 注射前 7 天开始连续 11 天给药。根据体重、绒毛高度、增殖细胞核抗原（PCNA）和核因子 kappa beta（NF-κB）的免疫组织化学表达、促炎因子白细胞介素 1β和肿瘤坏死因子α的水平以及髓过氧化物酶（MPO）浓度来评估肠黏膜炎。结果显示，与 5-FU 组相比，5-FU+B. infantis 组的体重和绒毛高度较高，PCNA 表达增加，NF-κB 和促炎因子表达减少，MPO 浓度降低。这些数据表明，益生菌 B. infantis 可有效减轻大鼠化疗诱导的肠黏膜炎。
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