采用气相色谱/质谱联用技术对体外受精反复失败患者的人卵泡液进行代谢组学分析。
Metabolomic profiling of human follicular fluid from patients with repeated failure of in vitro fertilization using gas chromatography/mass spectrometry.
作者信息
Xia Lan, Zhao Xiaoming, Sun Yun, Hong Yan, Gao Yuping, Hu Shuanggang
机构信息
Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics, Center for Reprodutive Medcine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University Shanghai, China.
出版信息
Int J Clin Exp Pathol. 2014 Sep 15;7(10):7220-9. eCollection 2014.
OBJECTIVE
To establish a gas chromatography/mass spectrometry (GC/MS)-based metabolomics method to compare the metabolites in the follicular fluid (FF) from patients with in vitro fertilization (IVF) and repeated IVF failure (RIF).
METHODS
A prospective study was employed in Center for Reprodutive Medcine, Renji Hospital, Shanghai, China, between January and October 2010. FF samples were collected from 13 patients with RIF and 15 patients who achieved pregnancy after the first IVF cycle.
RESULTS
Partial least squares (PLS) discriminant analysis of the PCA data revealed that the samples were scattered into two different regions. FF from the two groups differed with respect to 20 metabolites. FF from RIF group showed elevated levels of several amino acids (valine, threonine, isoleucine, cysteine, serine, proline, alanine, phenylalanine, lysine, methionine and ornithine), and reduced levels of dicarboxylic acids, cholesterol and some organic acids.
CONCLUSIONS
The studies corroborated successful determination of the levels of metabolite in the FF.
目的
建立一种基于气相色谱/质谱联用（GC/MS）的代谢组学方法，以比较体外受精（IVF）患者与反复体外受精失败（RIF）患者卵泡液（FF）中的代谢物。
方法
2010年1月至10月，在中国上海仁济医院生殖医学中心进行了一项前瞻性研究。收集了13例反复体外受精失败患者和15例首次体外受精周期后成功妊娠患者的卵泡液样本。
结果
对主成分分析（PCA）数据进行偏最小二乘（PLS）判别分析显示，样本分散在两个不同区域。两组卵泡液在20种代谢物方面存在差异。反复体外受精失败组的卵泡液中几种氨基酸（缬氨酸、苏氨酸、异亮氨酸、半胱氨酸、丝氨酸、脯氨酸、丙氨酸、苯丙氨酸、赖氨酸、蛋氨酸和鸟氨酸）水平升高，二羧酸、胆固醇和一些有机酸水平降低。
结论
这些研究证实了成功测定卵泡液中代谢物的水平。
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