Mortensen Martin Mørck, Høyer Søren, Orntoft Torben Falck, Sørensen Karina Dalsgaard, Dyrskjøt Lars, Borre Michael
Department of Molecular Medicine, Aarhus University Hospital, Brendstrupgaardsvej 100, 8200 Aarhus N, Denmark.
BMC Cancer. 2014 Nov 21;14:859. doi: 10.1186/1471-2407-14-859.
Prostate cancer is one of the leading causes of cancer death amongst men in economically advanced countries. The disease is characterized by a greatly varying clinical course, where some patients harbor non- or slowly-progressive disease, others highly aggressive disease. There is a great lack of markers to differentiate between aggressive and indolent disease. Markers that could help to identify patients needing curative treatment while sparing those who do not.
MicroRNA profiling of 672 microRNAs using multiplex RT-qPCR was performed using 36 prostate cancer samples to evaluate the association of microRNAs and biochemical recurrence after radical prostatectomy.
Among 31 microRNAs associated with recurrence, we identified miR-449b, which was further validated in an independent cohort of 163 radical prostatectomy patients. Patients expressing miR-449b had a significantly higher risk of recurrence (HR = 1.57; p = 0.028), and miR-449b was shown to be an independent predictor of recurrence after prostatectomy (HR = 1.9; p = 0.003) when modeled with known risk factors of recurrent disease in multivariate analysis.
High miR-449b expression was shown to be an independent predictor of biochemical recurrence after radical prostatectomy.
在经济发达国家，前列腺癌是男性癌症死亡的主要原因之一。该疾病的临床病程差异很大，一些患者患有非进展性或缓慢进展性疾病，另一些则患有高度侵袭性疾病。目前非常缺乏能够区分侵袭性疾病和惰性疾病的标志物。这些标志物有助于识别需要进行根治性治疗的患者，同时避免对不需要治疗的患者进行治疗。
使用36份前列腺癌样本，通过多重逆转录定量聚合酶链反应对672种微小RNA进行分析，以评估微小RNA与前列腺癌根治术后生化复发之间的关联。
在与复发相关的31种微小RNA中，我们鉴定出了miR-449b，并在一个由163名接受前列腺癌根治术的患者组成的独立队列中对其进行了进一步验证。表达miR-449b的患者复发风险显著更高（风险比=1.57；p=0.028），在多变量分析中，当将miR-449b与已知的复发疾病风险因素进行建模时，它被证明是前列腺癌根治术后复发的独立预测因子（风险比=1.9；p=0.003）。
高miR-449b表达被证明是前列腺癌根治术后生化复发的独立预测因子。
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