透过 X 射线晶体学瞥见结构生物学。
A glimpse of structural biology through X-ray crystallography.
机构信息
Center for Structural Biology, Tsinghua-Peking Joint Center for Life Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China.
出版信息
Cell. 2014 Nov 20;159(5):995-1014. doi: 10.1016/j.cell.2014.10.051.
Since determination of the myoglobin structure in 1957, X-ray crystallography, as the anchoring tool of structural biology, has played an instrumental role in deciphering the secrets of life. Knowledge gained through X-ray crystallography has fundamentally advanced our views on cellular processes and greatly facilitated development of modern medicine. In this brief narrative, I describe my personal understanding of the evolution of structural biology through X-ray crystallography-using as examples mechanistic understanding of protein kinases and integral membrane proteins-and comment on the impact of technological development and outlook of X-ray crystallography.
自 1957 年肌红蛋白结构的测定以来，X 射线晶体学作为结构生物学的基础工具，在破解生命奥秘方面发挥了重要作用。通过 X 射线晶体学获得的知识从根本上推动了我们对细胞过程的认识，并极大地促进了现代医学的发展。在这篇简要的叙述中，我通过使用蛋白激酶和整合膜蛋白的机制理解的例子，描述了我对 X 射线晶体学的结构生物学演变的个人理解，并对技术发展的影响和 X 射线晶体学的展望进行了评论。
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