Eom Dae-Woon, Lee Ji Hwan, Kim Young-Joo, Hwang Gwi Seo, Kim Su-Nam, Kwak Jin Ho, Cheon Gab Jin, Kim Ki Hyun, Jang Hyuk-Jai, Ham Jungyeob, Kang Ki Sung, Yamabe Noriko
Department of Pathology, University of Ulsan College of Medicine, Gangneung Asan Hospital, Gangneung 25440, Korea.
Natural Products Research Institute, Korea Institute of Science and Technology, Gangneung 25451, Korea.
BMB Rep. 2015 Aug;48(8):461-6. doi: 10.5483/bmbrep.2015.48.8.216.
Epigallocatechin gallate (EGCG) and curcumin are well known to naturally-occurring anticancer agents. The aim of this study was to verify the combined beneficial anticancer effects of curcumin and EGCG on PC3 prostate cancer cells, which are resistant to chemotherapy drugs and apoptosis inducers. EGCG showed weaker inhibitory effect on PC3 cell proliferation than two other prostate cancer cell lines, LNCaP and DU145. Co-treatment of curcumin improved antiproliferative effect of EGCG on PC3 cells. The protein expressions of p21 were significantly increased by the co-treatment of EGCG and curcumin, whereas it was not changed by the treatment with each individual compound. Moreover, treatments of EGCG and curcumin arrested both S and G2/M phases of PC3 cells. These results suggest that the enhanced inhibitory effect of EGCG on PC3 cell proliferation by curcumin was mediated by the synergic up-regulation of p21-induced growth arrest and followed cell growth arrest.
表没食子儿茶素没食子酸酯（EGCG）和姜黄素是众所周知的天然抗癌剂。本研究的目的是验证姜黄素和EGCG对PC3前列腺癌细胞的联合有益抗癌作用，PC3前列腺癌细胞对化疗药物和凋亡诱导剂具有抗性。EGCG对PC3细胞增殖的抑制作用比其他两种前列腺癌细胞系LNCaP和DU145弱。姜黄素联合处理可提高EGCG对PC3细胞的抗增殖作用。EGCG和姜黄素联合处理可显著增加p21的蛋白表达，而单独使用每种化合物处理则无变化。此外，EGCG和姜黄素处理使PC3细胞的S期和G2/M期均停滞。这些结果表明，姜黄素增强EGCG对PC3细胞增殖的抑制作用是通过p21诱导的生长停滞的协同上调介导的，随后细胞生长停滞。
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