从微藻油中生产富含不饱和脂肪酸的新型结构三酰基甘油。
From microalgae oil to produce novel structured triacylglycerols enriched with unsaturated fatty acids.
机构信息
College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China; Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang 212018, PR China.
College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, PR China.
出版信息
Bioresour Technol. 2015 May;184:405-414. doi: 10.1016/j.biortech.2014.09.133. Epub 2014 Oct 6.
Novel structured triacylglycerols (STAGs) enriched with unsaturated fatty acids (UFAs) and low palmitic acid (PA) content were firstly synthesized from Schizochytrium sp. oil and oleic acid (OA) via solvent-free acidolysis catalyzed by Lipozyme RM IM. The results indicated that, the PA content decreased from 24.49% to 6.95%, while the UFAs content increased from 70.20% to 90.9% at the sn-1,3 positions in the STAGs under the optimal condition (i.e., lipase load of 7%, molar ratio of microalgae oil TAGs to OA of 1:3, and temperature of 65 °C). The lipase Lipozyme RM IM could be reused 16 times without significant loss of activity. The improved plastic and storage ranges of STAGs are useful for infant formula formulations, by which a possible method is blending of this product and 1,3-dioleoyl-2-palmitoylglycerol enriched fats and minor lipids based on the corresponding chemical compositions of human milk fat.
新型结构三酰基甘油（STAG）富含不饱和脂肪酸（UFA）和低棕榈酸（PA）含量，首次通过无溶剂酸解反应，由裂殖壶菌油和油酸（OA）在脂肪酶 Lipozym RM IM 的催化作用下合成。结果表明，在最佳条件下（即脂肪酶用量为 7%，微藻油三酰基甘油与 OA 的摩尔比为 1:3，温度为 65°C），STAGs 中 sn-1,3 位的 PA 含量从 24.49%降至 6.95%，而 UFA 含量从 70.20%增至 90.9%。脂肪酶 Lipozym RM IM 可以重复使用 16 次而没有明显的失活。STAGs 的改进的塑性和储存范围对婴儿配方奶粉的配方有用，通过该产品和富含 1,3-二油酰基-2-棕榈酰甘油的脂肪以及基于人乳脂肪的相应化学成分的少量脂质的混合，可以实现这一目标。
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