一种在昆虫介体中同时检测水稻条纹病毒和水稻黑条矮缩病毒的简化方法。
A simplified method for simultaneous detection of Rice stripe virus and Rice black-streaked dwarf virus in insect vector.
作者信息
Li Shuo, Wang Xi, Xu Jianxiang, Ji Yinghua, Zhou Yijun
机构信息
Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, China.
Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Jiangsu Technical Service Center of Diagnosis and Detection for Plant Virus Diseases, Nanjing 210014, China; College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
出版信息
J Virol Methods. 2015 Jan;211:32-5. doi: 10.1016/j.jviromet.2014.10.006. Epub 2014 Oct 25.
Rice stripe virus (RSV) and Rice black-streaked dwarf virus (RBSDV) are transmitted by their common vector small brown planthopper (SBPH) that cause serious crop losses in China. A simple reverse transcription-PCR method was developed for the simultaneous detection of RSV and RBSDV in single SBPH. Three primers targeted to RSV-RNA4 and RBSDV-S2 segments were designed to amplify respectively 1114-bp and 414-bp fragments in a reaction. The method is reliable, rapid and inexpensive for detecting the two viruses in vector, which could facilitate better forecasting and control of the virus diseases. Using this method, it was found that SBPH could carry RSV and RBSDV simultaneously.
水稻条纹病毒（RSV）和水稻黑条矮缩病毒（RBSDV）通过其共同传毒介体灰飞虱（SBPH）传播，这两种病毒在中国造成严重的作物损失。开发了一种简单的逆转录PCR方法，用于在单个灰飞虱中同时检测RSV和RBSDV。设计了针对RSV-RNA4和RBSDV-S2片段的3条引物，以便在一个反应中分别扩增出1114bp和414bp的片段。该方法用于检测介体中的这两种病毒可靠、快速且成本低廉，有助于更好地预测和控制病毒病害。利用该方法发现，灰飞虱可同时携带RSV和RBSDV。
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