Cao Ji-Liang, Wei Jin-Chao, Chen Mei-Wan, Su Huan-Xing, Wan Jian-Bo, Wang Yi-Tao, Li Peng
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa 999078, Macau.
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa 999078, Macau.
J Chromatogr A. 2014 Dec 5;1371:1-14. doi: 10.1016/j.chroma.2014.10.078. Epub 2014 Oct 31.
For the purpose of better understanding the complex Chinese herbal medicines (CHMs) and controlling their quality, powerful analytical techniques are essential. Although conventional one-dimensional (1D) chromatographic approaches have been widely used for the analysis of multiple components in CHMs, the complexity of CHM samples often exceeds the maximal capacity of any single separation mode. Therefore, in past decades, many researchers have attempted to explore the coupling of independent separation techniques to improve the resolving power for complex CHM samples. Two-dimensional (2D) separation systems, based on two independent columns with different separation mechanisms, have proven to be more powerful than 1D techniques and have been used successfully to separate and analyze CHM samples with excellent performance. This article aims to review the most recent advances in the strategies for analyzing CHMs using 2D chromatography. For this purpose, some remarkable applications of the commonly used couplings, mainly including 2D-GC and 2D-LC for analysis of CHMs, are described. Moreover, their major advantages and shortcomings are discussed, which might be helpful to the researchers who focus on quality control of CHMs.
为了更好地理解复杂的中药并控制其质量，强大的分析技术至关重要。尽管传统的一维（1D）色谱方法已广泛用于中药中多种成分的分析，但中药样品的复杂性常常超出任何单一分离模式的最大容量。因此，在过去几十年中，许多研究人员试图探索独立分离技术的联用，以提高对复杂中药样品的分离能力。基于具有不同分离机制的两根独立色谱柱的二维（2D）分离系统，已被证明比一维技术更强大，并已成功用于分离和分析中药样品，表现出色。本文旨在综述二维色谱分析中药策略的最新进展。为此，描述了常用联用方式的一些显著应用，主要包括用于中药分析的二维气相色谱（2D-GC）和二维液相色谱（2D-LC）。此外，还讨论了它们的主要优点和缺点，这可能对专注于中药质量控制的研究人员有所帮助。
