Barron A L, Menna J H, Moses E B, Rank R G, Ryu H, White H J
Department of Microbiology, University of Arkansas for Medical Sciences, Little Rock 72205.
Sex Transm Dis. 1989 Jan-Mar;16(1):41-6. doi: 10.1097/00007435-198901000-00009.
Female guinea pigs were inoculated intravaginally with guinea pig cytomegalovirus (GPCMV) propagated either in guinea pig embryo fibroblast cultures (GPEF) or salivary glands. The incidence of infection was higher with GPEF virus. Rare instances of isolation of GPCMV from cervical swabs 9-48 hr after inoculation was attributed to survival of inoculum in the genital tract. Neither immunofluorescence microscopy nor histopathologic examination showed evidence for active infection of genital tract tissue examined up to day 5 after inoculation. At necropsy on days 30-49, GPCMV was isolated from salivary glands and occasionally from pancreas and lymph nodes. Seroconversion following intravaginal inoculation was demonstrated by an enzyme-linked immunosorbent assay (ELISA) test, and titers were comparable to those after intraperitoneal or subcutaneous inoculation. However, titers of neutralizing antibody, determined by plaque-reduction assay, were significantly lower in the group inoculated intravaginally. These findings are relevant to consideration of cytomegalovirus as a sexually transmitted agent in humans.
将豚鼠巨细胞病毒（GPCMV）分别接种于在豚鼠胚胎成纤维细胞培养物（GPEF）或唾液腺中繁殖的雌性豚鼠阴道内。用GPEF病毒感染的发生率更高。接种后9 - 48小时从宫颈拭子中罕见地分离出GPCMV，这归因于接种物在生殖道中的存活。在接种后长达5天的时间里，免疫荧光显微镜检查和组织病理学检查均未显示所检查的生殖道组织有活跃感染的证据。在第30 - 49天尸检时，从唾液腺中分离出GPCMV，偶尔也从胰腺和淋巴结中分离出。通过酶联免疫吸附测定（ELISA）试验证明阴道内接种后发生了血清转化，其滴度与腹腔内或皮下接种后的滴度相当。然而，通过蚀斑减少试验测定的中和抗体滴度在阴道内接种组中显著较低。这些发现与将巨细胞病毒视为人类性传播病原体的考虑相关。
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