A highly sensitive protocol for the determination of Hg(2+) in environmental water using time-gated mode.

In this paper, a sensitive time-gated fluorescent sensing strategy for mercury ions (Hg(2+)) monitoring is developed based on Hg(2+)-mediated thymine (T)-Hg(2+)-T structure and the mechanism of fluorescence resonance energy transfer from Mn-doped CdS/ZnS quantum dots to graphene oxide. The authors employ two T-rich single-stranded DNA (ssDNA) as the capture probes for Hg(2+), and one of them is modified with Mn-doped CdS/ZnS quantum dots. The addition of Hg(2+) makes the two T-rich ssDNA hybrids with each other to form stable T-Hg(2+)-T coordination chemistry, which makes Mn-doped CdS/ZnS quantum dots far away from the surface of graphene oxide. As a result, the fluorescence signal is increased obviously compared with that without Hg(2+). The time-gated fluorescence intensities are linear with the concentrations of Hg(2+) in the range from 0.20 to 10 nM with a limit of detection of 0.11 nM. The detection limit is much lower than the U.S. Environmental Protection Agency limit of the concentration of Hg(2+) for drinking water. The time-gated fluorescent sensing strategy is specific for Hg(2+) even with interference by other metal ions based on the results of selectivity experiments. Importantly, the proposed sensing strategy is applied successfully to the determination of Hg(2+) in environmental water samples.