Boretti Vanessa Stolf, Corrêa Renata Nunes, dos Santos Silvana Soléo Ferreira, Leão Mariella Vieira Pereira, Gonçalves e Silva Célia Regina
Universidade de Taubaté (UNITAU), Taubaté, SP, Brasil.
Universidade de Taubaté (UNITAU), Taubaté, SP, Brasil.
Rev Paul Pediatr. 2014 Sep;32(3):151-6. doi: 10.1590/0103-0582201432301. Epub 2014 Oct 3.
: To evaluate the presence of microorganisms of the genus and on toys in the playroom of a teaching hospital, as well to as analyze the antimicrobial resistance from isolated strains.
: Samples were collected from 60 toys, using wet swabs, soon after being used by the children. The samples were inoculated in enriched and selective agar for isolation and later identification of the microorganisms. Antibiogram testing was performed by agar diffusion technique.
: The genus was present in 87.0% (52/60) of the toys. Seventy-three strains were isolated, with 29.0% (21/73) coagulase-positive and 71.0% (52/73) coagulasenegative. Among the coagulase-negative strains, 90.4% were resistant to penicillin, 65.4% to oxacillin, 28.8% to clarithromycin, 61.5% to clindamycin, and none to vancomycin. Among the coagulase-positive strains, 76.2% were resistant to penicillin, 23.8% to oxacillin, 23.8% to clarithromycin, 47.6% to clindamycin, and none to vancomycin. The genus was not detected in any of the evaluated toys.
: Toys can be contaminated with potentially pathogenic bacteria with antimicrobial resistance, representing a possible source of nosocomial infection for patients who are already debilitated.
评估教学医院游戏室玩具上葡萄球菌属和链球菌属微生物的存在情况,并分析分离菌株的抗菌药物耐药性。
在儿童使用玩具后立即用湿棉签从60个玩具上采集样本。将样本接种于富集和选择性琼脂中以分离并随后鉴定微生物。采用琼脂扩散技术进行药敏试验。
87.0%(52/60)的玩具上存在葡萄球菌属。共分离出73株菌株,其中凝固酶阳性的占29.0%(21/73),凝固酶阴性的占71.0%(52/73)。在凝固酶阴性菌株中,90.4%对青霉素耐药,65.4%对苯唑西林耐药,28.8%对克拉霉素耐药,61.5%对克林霉素耐药,对万古霉素均不耐药。在凝固酶阳性菌株中,76.2%对青霉素耐药,23.8%对苯唑西林耐药,23.8%对克拉霉素耐药,47.6%对克林霉素耐药,对万古霉素均不耐药。在所评估的玩具中未检测到链球菌属。
玩具可能被具有抗菌药物耐药性的潜在病原菌污染,这对本就虚弱的患者来说可能是医院感染的一个潜在来源。