霍乱弧菌mutL和mutS基因的克隆与特性分析：mutL基因的核苷酸序列

Cloning and characterization of mutL and mutS genes of Vibrio cholerae: nucleotide sequence of the mutL gene.

作者信息

Bera T K, Ghosh S K, Das J

机构信息

Biophysics Division, Indian Institute of Chemical Biology, Calcutta.

出版信息

Nucleic Acids Res. 1989 Aug 11;17(15):6241-51. doi: 10.1093/nar/17.15.6241.

DOI:10.1093/nar/17.15.6241

PMID:2549510

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC318275/

Abstract

The mutL and mutS genes of Vibrio cholerae have been identified using interspecific complementation of Escherichia coli mutL and mutS mutants with plasmids containing the gene bank of V. cholerae. The recombinant plasmid pJT470, containing a 4.7 kb fragment of V. cholerae DNA codes for a protein of molecular weight 92,000. The product of this gene reduces the spontaneous mutation frequency of the E. coli mutS mutant. The plasmid, designated pJT250, containing a 2.5 kb DNA fragment of V. cholerae and coding for a protein of molecular weight 62,000, complements the mutL gene function of E. coli mutL mutants. These gene products are involved in the repair of mismatches in DNA. The complete nucleotide sequence of mutL gene of V. cholerae has been determined.

摘要

利用含有霍乱弧菌基因文库的质粒对大肠杆菌mutL和mutS突变体进行种间互补，已鉴定出霍乱弧菌的mutL和mutS基因。重组质粒pJT470含有一段4.7kb的霍乱弧菌DNA片段，编码一种分子量为92,000的蛋白质。该基因的产物降低了大肠杆菌mutS突变体的自发突变频率。质粒pJT250含有一段2.5kb的霍乱弧菌DNA片段，编码一种分子量为62,000的蛋白质，可互补大肠杆菌mutL突变体的mutL基因功能。这些基因产物参与DNA错配修复。已确定霍乱弧菌mutL基因的完整核苷酸序列。