外周血单个核细胞中的微小RNA表达谱分析：慢性丙型肝炎潜在的诊断生物标志物
MicroRNA expression profiling in PBMCs: a potential diagnostic biomarker of chronic hepatitis C.
作者信息
Chang Chiu-Chun, Lin Chun-Che, Hsieh Wan-Ling, Lai Hsin-Wu, Tsai Chia-Hsun, Cheng Ya-Wen
机构信息
Institute of Medicine, Chung Shan Medical University, Taichung, Taiwan ; Division of Gastroenterology, Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan.
Division of Gastroenterology, Department of Internal Medicine, Chung Shan Medical University Hospital, Taichung, Taiwan ; School of Medicine, Chung Shan Medical University, Taichung, Taiwan.
出版信息
Dis Markers. 2014;2014:367157. doi: 10.1155/2014/367157. Epub 2014 Nov 18.
The expression levels of miR-16, miR-193b, miR-199a, miR-222, and miR-324 in PBMCs were significantly higher in CHC patients compared with healthy controls and significantly different between CHC patients with HCV genotype 1 (GT-1) and non-genotype-1 (non-GT-1). Multivariate logistic regression analysis also showed that patients with high expression levels of the six target miRNAs had an approximately 7.202-fold risk of CHC compared with those with low expression levels of the target miRNAs. We concluded that the expression levels of miR-16, miR-193b, miR-199a, miR-222, and miR-324 target miRNAs in PBMCs of CHC may act as significant risk biomarkers for the development of CHC.
与健康对照相比，慢性丙型肝炎（CHC）患者外周血单核细胞（PBMCs）中miR-16、miR-193b、miR-199a、miR-222和miR-324的表达水平显著更高，且在丙型肝炎病毒1型（GT-1）的CHC患者与非1型（非GT-1）的CHC患者之间存在显著差异。多因素逻辑回归分析还显示，与靶标miRNAs低表达水平的患者相比，六种靶标miRNAs高表达水平的患者患CHC的风险约高7.202倍。我们得出结论，CHC患者PBMCs中miR-16、miR-193b、miR-199a、miR-222和miR-324靶标miRNAs的表达水平可能是CHC发生的重要风险生物标志物。
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