蓖麻毒素和相思豆毒素并不抑制氨酰tRNA在延伸因子1依赖下与核糖体的结合。
Ricin and modeccin do not inhibit the elongation factor 1-dependent binding of aminoacyl-tRNA to ribosomes.
作者信息
Sperti S, Montanaro L
出版信息
Biochem J. 1979 Jan 15;178(1):233-6. doi: 10.1042/bj1780233.
Abstract
Ricin and modeccin do not affect the total number of ribosomes to which phenylalanyl-tRNA becomes bound in the EF 1-dependent reaction. Previous inconsistencies resulted from the use of the nitrocellulose-filter technique, which overestimates the number of control ribosomes engaged in the binding reaction if trace amounts of EF 2 contaminate the ribosomal preparations.
摘要
蓖麻毒素和相思豆毒素不会影响在依赖于EF 1的反应中与苯丙氨酰-tRNA结合的核糖体总数。以前的不一致结果是由于使用了硝酸纤维素滤膜技术，如果痕量的EF 2污染核糖体制剂，该技术会高估参与结合反应的对照核糖体数量。
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本文引用的文献
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
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Ricin - a potent inhibitor of protein synthesis.蓖麻毒素——一种强大的蛋白质合成抑制剂。
FEBS Lett. 1972 Feb 15;20(3):327-329. doi: 10.1016/0014-5793(72)80098-x.
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A supernatant factor involved in initiation complex formation with eukaryotic ribosomes.一种参与与真核核糖体形成起始复合物的上清因子。
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Inhibition by ricin of protein synthesis in vitro. Ribosomes as the target of the toxin.蓖麻毒素对体外蛋白质合成的抑制作用。核糖体作为毒素的作用靶点。
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Effect of development on the translation of messenger RNA in Artemia salina embryos.发育对卤虫胚胎中信使核糖核酸翻译的影响。
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Inhibition by ricin of protein synthesis in vitro: 60 S ribosomal subunit as the target of the toxin.蓖麻毒素对体外蛋白质合成的抑制作用：60S核糖体亚基作为毒素的作用靶点。
Biochem J. 1973 Nov;136(3):813-5. doi: 10.1042/bj1360813.
7
The interaction of Ricinus communis agglutinin with normal and tumor cell surfaces.蓖麻凝集素与正常细胞及肿瘤细胞表面的相互作用。
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8
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Biochemistry. 1974 Jan 1;13(1):196-204. doi: 10.1021/bi00698a029.
9
The binding of tritiated elongation-factors 1 and 2 to ribosomes from Krebs II mouse ascites-tumore cells. The influence of various antibiotics and toxins.氚标记的延伸因子1和2与克氏Ⅱ型小鼠腹水瘤细胞核糖体的结合。各种抗生素和毒素的影响。
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