Sperti S, Montanaro L
Biochem J. 1979 Jan 15;178(1):233-6. doi: 10.1042/bj1780233.
Ricin and modeccin do not affect the total number of ribosomes to which phenylalanyl-tRNA becomes bound in the EF 1-dependent reaction. Previous inconsistencies resulted from the use of the nitrocellulose-filter technique, which overestimates the number of control ribosomes engaged in the binding reaction if trace amounts of EF 2 contaminate the ribosomal preparations.
蓖麻毒素和相思豆毒素不会影响在依赖于EF 1的反应中与苯丙氨酰-tRNA结合的核糖体总数。以前的不一致结果是由于使用了硝酸纤维素滤膜技术,如果痕量的EF 2污染核糖体制剂,该技术会高估参与结合反应的对照核糖体数量。