miR-22 在食管鳞状细胞癌中下调，抑制细胞迁移和侵袭。
miR-22 is down-regulated in esophageal squamous cell carcinoma and inhibits cell migration and invasion.
机构信息
Department of Oncology, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, Xiangyang, 441000 China.
Department of Dermatology, Xiangyang Central Hospital, Affiliated Hospital of Hubei University of Arts and Science, 136 Jingzhou Street, Xiangyang, 441000 China.
出版信息
Cancer Cell Int. 2014 Dec 12;14(1):138. doi: 10.1186/s12935-014-0138-0. eCollection 2014.
BACKGROUND
Esophageal squamous cell carcinoma (ESCC) is one of the most common and deadly forms of cancer. Despite advances in the diagnosis and treatment of this cancer, the survival rate at five years is poor. Lately, miR-22 is identified as a tumor-suppressing microRNA in many human cancers. However, the specific function of miR-22 in ESCC is unclear at this point.
METHODS
We first measured miR-22 expression level in 30 paired of ESCC and matched normal tissues, ESCC cell lines by real-time quantitative RT-PCR. Invasion assay, MTT proliferation assay and wound-healing assay were performed to test the invasion and proliferation of ESCC cell after overexpression of miR-22.
RESULTS
We found that the expression of miR-22 in ESCC tissues and cell lines were much lower than that in normal control, respectively. The expression of miR-22 was inversely correlated with ESCC metastatic ability. Furthermore, transfection of miR-22 expression plasmid could significantly inhibit the cell proliferation, migration and invasion in Eca109 and Kyse410 ESCC cell lines.
CONCLUSIONS
Our findings suggest that miR-22 act as tumor suppressor and inhibiting ESCC cell migration and invasion. The findings of this study contribute to the current understanding of the functions of miR-22 in ESCC.
背景
食管鳞状细胞癌（ESCC）是最常见和最致命的癌症之一。尽管在这种癌症的诊断和治疗方面取得了进展，但五年生存率仍然很差。最近，miR-22 被确定为许多人类癌症中的一种肿瘤抑制 microRNA。然而，miR-22 在 ESCC 中的具体功能目前尚不清楚。
方法
我们首先通过实时定量 RT-PCR 测量了 30 对 ESCC 及匹配的正常组织和 ESCC 细胞系中 miR-22 的表达水平。通过侵袭实验、MTT 增殖实验和划痕愈合实验，检测过表达 miR-22 后 ESCC 细胞的侵袭和增殖能力。
结果
我们发现 miR-22 在 ESCC 组织和细胞系中的表达均明显低于正常对照。miR-22 的表达与 ESCC 的转移能力呈负相关。此外，转染 miR-22 表达质粒可显著抑制 Eca109 和 Kyse410 ESCC 细胞系的细胞增殖、迁移和侵袭。
结论
我们的研究结果表明，miR-22 作为肿瘤抑制因子，抑制 ESCC 细胞的迁移和侵袭。本研究的结果有助于当前对 miR-22 在 ESCC 中的功能的理解。
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