点带石斑鱼（Scatophagus argus）中FOXL2相对于Cyp19a基因的特征及性腺表达
Characterization and gonadal expression of FOXL2 relative to Cyp19a genes in spotted scat Scatophagus argus.
作者信息
Liu Huifen, Mu Xingjiang, Gui Lang, Su Maoliang, Li Hong, Zhang Guang, Liu Zhenhao, Zhang Junbin
机构信息
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.
出版信息
Gene. 2015 Apr 25;561(1):6-14. doi: 10.1016/j.gene.2014.12.060. Epub 2014 Dec 27.
In the present study, we cloned the full-length cDNAs of FOXL2, Cyp19a1a and Cyp19a1b and analyzed their expression patterns during gonadal development in spotted scat, Scatophagus argus. All three genes were expressed in ovaries and testes but showed sexual dimorphism. At early stages of gonadal development, the expression of FOXL2 in ovaries was higher than testes. FOXL2 expression deceased gradually as gonadal development continued, and reached the lowest level at the mature stage. Cyp19a1a and Cyp19a1b were expressed coordinately with FOXL2, except at the early vitellogenic stage in the ovary. The expression of FOXL2, Cyp19a1a and Cyp19a1b was mainly localized in granulosa cells of ovaries. In S. argus testes, strong expression of FOXL2 gene was observed in the interstitial cells including tubules and Leydig cells, while Cyp19a1a and Cyp19a1b were mainly expressed in Sertoli cells throughout gametogenesis. These results show that FOXL2 plays an essential role in sexual development, and imply that it may regulate Cyp19a1a and Cyp19a1b expression in S. argus.
在本研究中，我们克隆了FOXL2、Cyp19a1a和Cyp19a1b的全长cDNA，并分析了它们在点带石斑鱼性腺发育过程中的表达模式。这三个基因在卵巢和精巢中均有表达，但表现出性别二态性。在性腺发育早期，FOXL2在卵巢中的表达高于精巢。随着性腺发育的持续，FOXL2的表达逐渐降低，并在成熟阶段达到最低水平。Cyp19a1a和Cyp19a1b与FOXL2协同表达，但在卵巢早期卵黄生成阶段除外。FOXL2、Cyp19a1a和Cyp19a1b的表达主要定位于卵巢的颗粒细胞中。在点带石斑鱼精巢中，在包括小管和间质细胞在内的间质细胞中观察到FOXL2基因的强表达，而在整个配子发生过程中，Cyp19a1a和Cyp19a1b主要在支持细胞中表达。这些结果表明，FOXL2在性别发育中起重要作用，并暗示它可能调节点带石斑鱼中Cyp19a1a和Cyp19a1b的表达。
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