Kharrat Imen, Mezghani Maha, Casse Nathalie, Denis Françoise, Caruso Aurore, Makni Hanem, Capy Pierre, Rouault Jacques-Deric, Chénais Benoît, Makni Mohamed
Faculté des Sciences de Tunis, Université de Tunis El Manar, UR11ES10 Génomique des insectes ravageurs, 2092, Manar II, Tunisia.
Genetica. 2015 Feb;143(1):63-72. doi: 10.1007/s10709-014-9814-1. Epub 2015 Jan 3.
Mariner-like elements (MLEs) are Class II transposons present in all eukaryotic genomes in which MLEs have been searched for. This article reports the detection of MLEs in seven of the main fruit tree aphid species out of eight species studied. Deleted MLE sequences of 916-919 bp were characterized, using the terminal-inverted repeats (TIRs) of mariner elements belonging to the mauritiana Subfamily as primers. All the sequences detected were deleted copies of full-length elements that included the 3'- and 5'-TIRs but displayed internal deletions affecting Mos1 activity. Networks based on the mtDNA cytochrome oxidase subunit-I (CO-I) and MLE sequences were incongruent, suggesting that mutations in transposon sequences had accumulated before speciation of tree aphid species occurred, and that they have been maintained in this species via vertical transmissions. This is the first evidence of the widespread occurrence of MLEs in aphids.
水手样元件（MLEs）是已在其中搜索到MLEs的所有真核生物基因组中存在的II类转座子。本文报道了在所研究的8种主要果树蚜虫中，有7种检测到了MLEs。以属于mauritiana亚科的水手元件的末端反向重复序列（TIRs）为引物，对916 - 919 bp的缺失MLE序列进行了表征。检测到的所有序列都是全长元件的缺失拷贝，其中包括3'和5'TIRs，但显示出影响Mos1活性的内部缺失。基于线粒体DNA细胞色素氧化酶亚基I（CO-I）和MLE序列的网络不一致，这表明转座子序列中的突变在树蚜虫物种形成之前就已经积累，并且它们通过垂直传播在该物种中得以保留。这是MLEs在蚜虫中广泛存在的首个证据。
