转座子Tn916在蕈状支原体基因组中的插入位点位置。
Location of sites of transposon Tn916 insertion in the Mycoplasma mycoides genome.
作者信息
Whitley J C, Finch L R
机构信息
Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Australia.
出版信息
J Bacteriol. 1989 Dec;171(12):6870-2. doi: 10.1128/jb.171.12.6870-6872.1989.
Abstract
The genome of Mycoplasma mycoides subsp. mycoides GC1176-2 was cleaved into six large fragments by the endonuclease KpnI which also cleaved the transposon Tn916 once. This has allowed genomic mapping of insertion sites for 50 transformants of GC1176-2 containing Tn916. Almost all of the mapped sites were clearly separate. The transformants provide a bank of genomes each with a KpnI site at a different position to facilitate mapping of gene loci.
摘要
蕈状支原体丝状亚种GC1176 - 2的基因组被内切酶KpnI切割成六个大片段，该内切酶还切割转座子Tn916一次。这使得对含有Tn916的GC1176 - 2的50个转化体的插入位点进行基因组定位成为可能。几乎所有定位的位点都明显分开。这些转化体提供了一组基因组库，每个基因组在不同位置都有一个KpnI位点，便于基因座的定位。
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