Kim Jung-Eun, Kim Hyejin, An Seong Soo A, Maeng Eun Ho, Kim Meyoung-Kon, Song Yoon-Jae
Department of Life Science, Gachon University, Seongnam-Si, South Korea.
Department of Bionano Technology, Gachon University, Seongnam-Si, South Korea.
Int J Nanomedicine. 2014 Dec 15;9 Suppl 2(Suppl 2):235-41. doi: 10.2147/IJN.S57936. eCollection 2014.
Silicon dioxide (SiO2) and zinc oxide (ZnO) nanoparticles are widely used in various applications, raising issues regarding the possible adverse effects of these metal oxide nanoparticles on human cells. In this study, we determined the cytotoxic effects of differently charged SiO2 and ZnO nanoparticles, with mean sizes of either 100 or 20 nm, on the U373MG human glioblastoma cell line. The overall cytotoxicity of ZnO nanoparticles against U373MG cells was significantly higher than that of SiO2 nanoparticles. Neither the size nor the surface charge of the ZnO nanoparticles affected their cytotoxicity against U373MG cells. The 20 nm SiO2 nanoparticles were more toxic than the 100 nm nanoparticles against U373MG cells, but the surface charge had little or no effect on their cytotoxicity. Both SiO2 and ZnO nanoparticles activated caspase-3 and induced DNA fragmentation in U373MG cells, suggesting the induction of apoptosis. Thus, SiO2 and ZnO nanoparticles appear to exert cytotoxic effects against U373MG cells, possibly via apoptosis.
二氧化硅（SiO₂）和氧化锌（ZnO）纳米颗粒被广泛应用于各种领域，这引发了关于这些金属氧化物纳米颗粒对人体细胞可能产生的不利影响的问题。在本研究中，我们测定了平均尺寸为100或20纳米、带不同电荷的SiO₂和ZnO纳米颗粒对U373MG人胶质母细胞瘤细胞系的细胞毒性作用。ZnO纳米颗粒对U373MG细胞的总体细胞毒性显著高于SiO₂纳米颗粒。ZnO纳米颗粒的尺寸和表面电荷均不影响其对U373MG细胞的细胞毒性。20纳米的SiO₂纳米颗粒对U373MG细胞的毒性比100纳米的纳米颗粒更大，但表面电荷对其细胞毒性几乎没有影响。SiO₂和ZnO纳米颗粒均激活了U373MG细胞中的半胱天冬酶-3并诱导了DNA片段化，提示诱导了细胞凋亡。因此，SiO₂和ZnO纳米颗粒似乎对U373MG细胞发挥细胞毒性作用，可能是通过细胞凋亡实现的。
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