酿酒酵母SKI3基因的克隆与特性分析表明其与SKI5基因存在等位关系。
Cloning and characterization of the SKI3 gene of Saccharomyces cerevisiae demonstrates allelism to SKI5.
作者信息
Hougan L, Thomas D Y, Whiteway M
机构信息
Genetic Engineering Section, NRC Biotechnology Research Institute, Montreal, Quebec, Canada.
出版信息
Curr Genet. 1989 Sep;16(3):139-43. doi: 10.1007/BF00391469.
We have identified a mutant strain of the yeast Saccharomyces cerevisiae which overproduces killer toxin. This strain contains a single mutation which fails to complement defects in both the SKI3 and SKI5 genes, while a cloned copy of this gene complements both the ski3 and ski5 defects. The level of secreted toxin from a cDNA based plasmid is not increased in a ski3 strain, showing that the overproduction phenotype is dependent upon an increased level of M1 dsRNA.
我们已经鉴定出一种酿酒酵母的突变菌株，该菌株过量产生杀伤毒素。此菌株含有一个单一突变，该突变无法弥补SKI3和SKI5基因的缺陷，而该基因的克隆拷贝则可弥补ski3和ski5的缺陷。基于cDNA的质粒分泌的毒素水平在ski3菌株中并未增加，这表明过量生产表型取决于M1双链RNA水平的升高。
Zotero 插件