土曲霉中的有性重组。

Sexual recombination in Aspergillus tubingensis.

作者信息

Olarte Rodrigo A, Horn Bruce W, Singh Rakhi, Carbone Ignazio

机构信息

Center for Integrated Fungal Research, Department of Plant Pathology, North Carolina State University, Raleigh, North Carolina 27695.

National Peanut Research Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Dawson, Georgia 39842.

出版信息

Mycologia. 2015 Mar-Apr;107(2):307-12. doi: 10.3852/14-233. Epub 2015 Jan 8.

DOI:10.3852/14-233

PMID:25572097

Abstract

Aspergillus tubingensis from section Nigri (black Aspergilli) is closely related to A. niger and is used extensively in the industrial production of enzymes and organic acids. We recently discovered sexual reproduction in A. tubingensis, and in this study we demonstrate that the progeny are products of meiosis. Progeny were obtained from six crosses involving five MAT1-1 strains and two MAT1-2 strains. We examined three loci, including mating type (MAT), RNA polymerase II (RPB2) and β-tubulin (BT2), and found that 84% (58/69) of progeny were recombinants. Recombination associated with sexual reproduction in A. tubingensis provides a new option for the genetic improvement of industrial strains for enzyme and organic acid production.

摘要

来自黑曲霉组（黑色曲霉）的管囊曲霉与黑曲霉密切相关，并广泛用于酶和有机酸的工业生产。我们最近发现了管囊曲霉的有性繁殖，并且在本研究中我们证明后代是减数分裂的产物。后代来自涉及5个MAT1-1菌株和2个MAT1-2菌株的6次杂交。我们检测了三个位点，包括交配型（MAT）、RNA聚合酶II（RPB2）和β-微管蛋白（BT2），发现84%（58/69）的后代是重组体。与管囊曲霉有性繁殖相关的重组为酶和有机酸生产工业菌株的遗传改良提供了新的选择。