高效液相色谱-荧光检测法测定 Cannonau 和 Vermentino 葡萄酒中的丹磺酰化氨基酸和生物胺。
Determination of dansylated amino acids and biogenic amines in Cannonau and Vermentino wines by HPLC-FLD.
机构信息
Department of Life and Environmental Sciences, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy.
Department of Life and Environmental Sciences, University of Cagliari, Via Ospedale 72, 09124 Cagliari, Italy.
出版信息
Food Chem. 2015 May 15;175:29-35. doi: 10.1016/j.foodchem.2014.11.120. Epub 2014 Nov 27.
Free amino acids (AA) and biogenic amines (BA) were quantified for the first time in Cannonau and Vermentino wines, the two most popular "Controlled Designation of Origin" wines from Sardinia (Italy). An analytical method for the simultaneous determination of AA and BA was developed, using selective derivatization with dansyl chloride followed by HPLC with fluorescence detection. Thirty-two compounds were identified in the wines analysed. High levels of AA were found, with proline being the most abundant with average levels of 1244 ± 398 and 1008 ± 281 mg/L in Cannonau and Vermentino wines, respectively. BA were detected at average concentrations <10mg/L, except putrescine which reached 20.5 ± 10.2mg/L in Cannonau wines. Histamine was never detected in any Vermentino wines. γ-Aminobutyric acid, 4-hydroxyproline, glycine, leucine+isoleucine and putrescine proved to be useful for differentiating Cannonau wines from Vermentino wines.
首次定量测定了撒丁岛（意大利）两种最受欢迎的“法定产区”葡萄酒——卡诺娜（Cannonau）和维蒙蒂诺（Vermentino）中的游离氨基酸（AA）和生物胺（BA）。采用丹磺酰氯选择性衍生化，结合高效液相色谱荧光检测，建立了一种同时测定 AA 和 BA 的分析方法。对所分析的葡萄酒中 32 种化合物进行了鉴定。结果表明，葡萄酒中 AA 含量较高，其中脯氨酸含量最高，卡诺娜和维蒙蒂诺葡萄酒中的平均水平分别为 1244±398mg/L 和 1008±281mg/L。BA 的平均浓度<10mg/L，但在卡诺娜葡萄酒中检测到腐胺，平均浓度为 20.5±10.2mg/L。组胺从未在任何维蒙蒂诺葡萄酒中检出。γ-氨基丁酸、4-羟基脯氨酸、甘氨酸、亮氨酸+异亮氨酸和腐胺可用于区分卡诺娜葡萄酒和维蒙蒂诺葡萄酒。
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