Bonnart Remi, Waddell John, Haiby Kathy, Widrlenchner Mark P, Volk Gayle M
USDA-ARS National Center for Genetic Resources Preservation, Fort Collins, CO, USA.
Greenwood Resources, Portland, OR, USA.
Cryo Letters. 2014 Nov-Dec;35(6):507-15.
Methods are needed for the conservation of clonally maintained trees of Populus and Salix. In this work, Populus trichocarpa and Salix genetic resources were cryopreserved using dormant scions as the source explant.
We quantified the recovery of cryopreserved materials that originated from diverse field environments by using either direct sprouting or grafting.
Scions (either at their original moisture content of 48 to 60% or dried to 30%) were slowly cooled to -35 degree C, transferred to the vapor phase of liquid nitrogen (LNV, -160 degree C), and warmed before determining survival.
Dormant buds from P. trichocarpa clones from Westport and Boardman, OR had regrowth levels between 42 and 100%. Direct rooting of cryopreserved P. trichocarpa was also possible. Ten of 11 cryopreserved Salix accessions, representing 10 different species, exhibited at least 40% bud growth and rooting after 6 weeks when a bottom-heated rooting system was implemented.
We demonstrate that dormant buds of P. trichocarpa and Salix accessions can be cryopreserved and successfully regenerated without the use of tissue culture.
需要有方法来保存杨树和柳树的无性系维持树木。在这项工作中，以休眠接穗作为外植体来源，对毛果杨和柳树的遗传资源进行了冷冻保存。
我们通过直接发芽或嫁接来量化源自不同田间环境的冷冻保存材料的恢复情况。
将接穗（含水量为48%至60%或干燥至30%）缓慢冷却至-35℃，转移至液氮气相（LNV，-160℃），并在确定存活率之前进行解冻。
来自俄勒冈州韦斯特波特和博德曼的毛果杨无性系的休眠芽再生水平在42%至100%之间。冷冻保存的毛果杨也可以直接生根。当采用底部加热生根系统时，11个冷冻保存的柳树种质中有10个，代表10个不同物种，在6周后表现出至少40%的芽生长和生根。
我们证明了毛果杨和柳树种质的休眠芽可以在不使用组织培养的情况下进行冷冻保存并成功再生。
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