构建 pH 敏感的溶菌酶/果胶纳米凝胶用于肿瘤甲氨蝶呤递送。
Construction of pH-sensitive lysozyme/pectin nanogel for tumor methotrexate delivery.
机构信息
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Ministry of Education, China.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Ministry of Education, China.
出版信息
Colloids Surf B Biointerfaces. 2015 Feb 1;126:459-66. doi: 10.1016/j.colsurfb.2014.12.051. Epub 2015 Jan 6.
Novel nano-particles were developed from lysozyme-pectin through self-assembly, and the nanogels could be used as a carrier for the antitumor agent, methotrexate (MTX). The nanogels exhibited spherical with diameters about 109 ± 2 nm and narrow particle size distribution, as well as negative surface charge. Furthermore, the particle size and morphology of the nanogels hardly changed with the incorporation of MTX. The loading capacity of MTX in nanogels could reach 17.58 ± 0.85%. MTX-loaded nanogels were pH-dependent, accelerated release of MTX at a decreasing pH from 7.4 to 5.3. The MTT assay indicated that encapsulated MTX exhibited higher anticancer activity than free MTX. Meanwhile, MTX-loaded nanogels could be effectively endocytosed by HepG2 cells, resulting in enhanced cancer-cell apoptosis comparing to free MTX. It indicated that the nanogels had good biocompatibility and low toxicity. The obtained nanogels had great potential in the development of a new nanocarrier for anti-cancer drug delivery.
新型纳米颗粒是通过溶菌酶-果胶自组装开发的，纳米凝胶可用作抗肿瘤药物甲氨蝶呤（MTX）的载体。纳米凝胶呈球形，直径约为 109 ± 2nm，粒径分布较窄，带负电荷。此外，随着 MTX 的掺入，纳米凝胶的粒径和形态几乎没有变化。纳米凝胶对 MTX 的包载量可达 17.58 ± 0.85%。载 MTX 的纳米凝胶呈 pH 依赖性，在 pH 值从 7.4 降低至 5.3 时，MTX 加速释放。MTT 试验表明，包载的 MTX 比游离 MTX 具有更高的抗癌活性。同时，载 MTX 的纳米凝胶可被 HepG2 细胞有效内吞，与游离 MTX 相比，可促进癌细胞凋亡。这表明纳米凝胶具有良好的生物相容性和低毒性。所得纳米凝胶在开发用于抗癌药物递送的新型纳米载体方面具有很大的潜力。
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