Mao Zhong-Ping, Zhao Li-Jun, Zhou Shui-Hong, Liu Meng-Qin, Tan Wei-Feng, Yao Hong-Tian
Department of Otolaryngology, The Second Hospital of Shaoxing City, Shaoxing, Zhejiang 312000, P.R. China.
Department of Otolaryngology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China.
Oncol Lett. 2015 Feb;9(2):806-810. doi: 10.3892/ol.2014.2752. Epub 2014 Dec 1.
Increasing glucose transporter-1 (GLUT-1) activity is one of the most important ways to increase the cellular influx of glucose. We previously demonstrated that increased GLUT-1 expression was an independent predictor of survival in patients with laryngeal carcinoma. Thus, GLUT-1 may present a novel therapeutic target in laryngeal carcinoma. In this study, the expression of GLUT-1, P-glycoprotein (P-gp), multidrug resistance-associated protein 1 (MRP1) and glutathione S-transferase-π (GST-π) in laryngeal carcinomas was investigated by immunohistochemistry. Additionally, possible correlations between GLUT-1 and P-gp, MRP1 and GST-π and various clinicopathological parameters were analyzed. In this study, 52.9% (18/34), 58.8% (20/34), 20.6% (7/34) and 58.8% (20/34) of the laryngeal carcinomas were positive for GLUT-1, P-gp, MRP1 and GST-π, respectively. The expression of GLUT-1, P-gp, MRP1 and GST-π was higher in laryngeal carcinoma specimens when compared with laryngeal precancerous lesions (P<0.05). Pearson's correlation analysis showed correlations between GLUT-1 and P-gp (r=0.364; P=0.034), GLUT-1 and MRP1 (r=0.359; P=0.037) and P-gp and GST-π (r=0.426; P=0.012). GLUT-1 expression was found to significantly correlate with tumor-node-metastasis classification (P=0.02) and clinical stage (P=0.037). Furthermore, P-gp was found to significantly correlate with clinical stage (P=0.026). Univariate analysis showed that MRP1 expression was significantly associated with poor survival (c=5.16; P=0.023). Multivariate analysis revealed that lymph node metastasis (P=0.009) and MRP1 overexpression (P=0.023) were significant predictors of poor survival. In the present study, the expression of GLUT-1, P-gp, MRP1 and GST-π in laryngeal carcinomas was investigated, as well as the correlations between these proteins. P-gp was found to significantly correlate with clinical stage, while MRP1 overexpression was significantly associated with poor survival.
提高葡萄糖转运蛋白1（GLUT-1）的活性是增加细胞葡萄糖内流的最重要途径之一。我们之前证明，GLUT-1表达增加是喉癌患者生存的独立预测因素。因此，GLUT-1可能是喉癌的一个新的治疗靶点。在本研究中，采用免疫组织化学方法检测了喉癌中GLUT-1、P-糖蛋白（P-gp）、多药耐药相关蛋白1（MRP1）和谷胱甘肽S-转移酶π（GST-π）的表达。此外，分析了GLUT-1与P-gp、MRP1与GST-π之间可能的相关性以及各种临床病理参数。在本研究中，分别有52.9%（18/34）、58.8%（20/34）、20.6%（7/34）和58.8%（20/34）的喉癌GLUT-1、P-gp、MRP1和GST-π呈阳性。与喉癌前病变相比，喉癌标本中GLUT-1、P-gp、MRP1和GST-π的表达更高（P<0.05）。Pearson相关分析显示GLUT-1与P-gp（r=0.364；P=0.034）、GLUT-1与MRP1（r=0.359；P=0.037）以及P-gp与GST-π（r=0.426；P=0.012）之间存在相关性。发现GLUT-1表达与肿瘤-淋巴结-转移分类（P=0.02）和临床分期（P=0.037）显著相关。此外，发现P-gp与临床分期显著相关（P=0.026）。单因素分析显示MRP1表达与不良生存显著相关（c=5.16；P=0.023）。多因素分析显示淋巴结转移（P=0.009）和MRP1过表达（P=0.023）是不良生存的重要预测因素。在本研究中，检测了喉癌中GLUT-1、P-gp、MRP1和GST-π的表达以及这些蛋白之间的相关性；发现P-gp与临床分期显著相关，而MRP1过表达与不良生存显著相关。
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