Seki Tomohisa, Fukuda Keiichi
Tomohisa Seki, Keiichi Fukuda, Department of Cardiology, Keio University School of Medicine, Tokyo 160-8582, Japan.
World J Stem Cells. 2015 Jan 26;7(1):116-25. doi: 10.4252/wjsc.v7.i1.116.
Reprograming somatic cells using exogenetic gene expression represents a groundbreaking step in regenerative medicine. Induced pluripotent stem cells (iPSCs) are expected to yield novel therapies with the potential to solve many issues involving incurable diseases. In particular, applying iPSCs clinically holds the promise of addressing the problems of immune rejection and ethics that have hampered the clinical applications of embryonic stem cells. However, as iPSC research has progressed, new problems have emerged that need to be solved before the routine clinical application of iPSCs can become established. In this review, we discuss the current technologies and future problems of human iPSC generation methods for clinical use.
利用外源性基因表达对体细胞进行重编程是再生医学领域的一个开创性步骤。诱导多能干细胞(iPSC)有望带来新的治疗方法,有可能解决许多涉及不治之症的问题。特别是,将iPSC应用于临床有望解决免疫排斥和伦理问题,这些问题一直阻碍着胚胎干细胞的临床应用。然而,随着iPSC研究的进展,出现了一些新问题,在iPSC常规临床应用得以确立之前需要加以解决。在这篇综述中,我们讨论了用于临床的人类iPSC生成方法的当前技术和未来问题。