Töpfer R, Gronenborn B, Schell J, Steinbiss H H
Max-Planck-Institut für Züchtungsforschung, Abteilung Genetische Grundlagen der Pflanzenzüchtung, Köln, Federal Republic of Germany.
Plant Cell. 1989 Jan;1(1):133-9. doi: 10.1105/tpc.1.1.133.
Uptake of DNA in dry and viable embryos of wheat by imbibition in DNA solution was detected by monitoring the transient expression of chimeric genes. Gene expression vectors used in this study contained a neomycin phosphotransferase (NPT) II reporter gene fused to various promoters. Some of the chimeric "neo" genes were shown to yield reproducibly NPT II activity in germinating embryos. This NPT II activity was increased markedly when the neo genes were carried by a vector capable of autonomous replication. Dimers of wheat dwarf virus, a monopartite gemini virus, were thus shown to be effective in amplifying the transient expressed NPT II activity in embryos of several cereals. These and other observations indicate that the observed transient expression really results from DNA uptake and expression in plant embryo cells and is not due to contaminating microorganisms.
通过监测嵌合基因的瞬时表达,检测了小麦干燥且有活力的胚在DNA溶液中吸胀时对DNA的摄取情况。本研究中使用的基因表达载体包含一个与各种启动子融合的新霉素磷酸转移酶(NPT)II报告基因。一些嵌合“新”基因在萌发的胚中可重复产生NPT II活性。当新基因由能够自主复制的载体携带时,这种NPT II活性显著增加。因此,单分体双生病毒小麦矮缩病毒的二聚体被证明可有效放大几种谷物胚中瞬时表达的NPT II活性。这些以及其他观察结果表明,观察到的瞬时表达确实是植物胚细胞摄取和表达DNA的结果,而非由于污染微生物所致。