细胞死亡过程中线粒体功能障碍的分析。

Analysis of mitochondrial dysfunction during cell death.

作者信息

Gogvadze Vladimir, Orrenius Sten, Zhivotovsky Boris

机构信息

Division of Toxicology, Institute of Environmental Medicine, Karolinska Institutet, 210, Stockholm, 171 77, Sweden,

出版信息

Methods Mol Biol. 2015;1264:385-93. doi: 10.1007/978-1-4939-2257-4_33.

DOI:10.1007/978-1-4939-2257-4_33

PMID:25631030

Abstract

Mitochondria play a key role in various modes of cell death. Analysis of mitochondrial dysfunction and the release of proteins from the intermembrane space of mitochondria represent essential tools in cell death investigation. Here we describe how to evaluate release of intermembrane space proteins during apoptosis, alterations in the mitochondrial membrane potential, and oxygen consumption in apoptotic cells.

摘要

线粒体在多种细胞死亡模式中发挥关键作用。分析线粒体功能障碍以及线粒体外膜间隙蛋白的释放是细胞死亡研究中的重要手段。在此，我们描述如何评估凋亡过程中膜间隙蛋白的释放、线粒体膜电位的变化以及凋亡细胞中的耗氧量。