阔叶拉塞草（Laserpitium latifolium L.）和奥赫里德湖拉塞草（Laserpitium ochridanum Micevski）（伞形科）精油的成分及抗菌活性

Composition and antimicrobial activity of the essential oils of Laserpitium latifolium L. and L. ochridanum Micevski (Apiaceae).

作者信息

Popović Višnja B, Petrović Silvana D, Milenković Marina T, Drobac Milica M, Couladis Maria A, Niketić Marjan S

机构信息

University of Belgrade, Faculty of Pharmacy, Department of Pharmacognosy, V. Stepe 450, RS-11221 Belgrade (phone: +381-11-3951319; fax: +381-11-3972840); Ghent University, Faculty of Science, Laboratory for Organic and Bio-organic Synthesis, Department of Organic and Macromolecular Chemistry, Krijgslaan 281 S4, BE-9000 Ghent, (phone: +32-9-2644476; fax: +32-9-2644998).

出版信息

Chem Biodivers. 2015 Jan;12(1):170-7. doi: 10.1002/cbdv.201400127.

DOI:10.1002/cbdv.201400127

PMID:25641845

Abstract

The chemical composition and antimicrobial activity of essential oils of Laserpitium latifolium and L. ochridanum were investigated. The essential oils were isolated by steam distillation and characterized by GC-FID and GC/MS analyses. All essential oils were distinguished by high contents of monoterpenes, and α-pinene was the most abundant compound in the essential oils of L. latifolium underground parts and fruits (contents of 44.4 and 44.0%, resp.). The fruit essential oil was also rich in sabinene (26.8%). Regarding the L. ochridanum essential oils, the main constituents were limonene in the fruit oil (57.7%) and sabinene in the herb oil (25.9%). The antimicrobial activity of these essential oils as well as that of L. ochridanum underground parts, whose composition was reported previously, was tested by the broth-microdilution method against four Gram-positive and three Gram-negative bacteria and two Candida albicans strains. Except the L. latifolium underground-parts essential oil, the other investigated oils showed a high antimicrobial potential against Staphylococcus aureus, S. epidermidis, Micrococcus luteus, or Candida albicans (minimal inhibitory concentrations of 13.0-73.0 μg/ml), comparable to or even higher than that of thymol, which was used as reference compound.

摘要

对宽叶岩风（Laserpitium latifolium）和奥赫里德岩风（L. ochridanum）精油的化学成分和抗菌活性进行了研究。通过水蒸气蒸馏法分离精油，并采用气相色谱 - 火焰离子化检测（GC - FID）和气相色谱 - 质谱联用（GC/MS）分析对其进行表征。所有精油的特点是单萜类化合物含量高，α - 蒎烯是宽叶岩风地下部分和果实精油中含量最高的化合物（分别为44.4%和44.0%）。果实精油中也富含桧烯（26.8%）。对于奥赫里德岩风精油，果实精油的主要成分是柠檬烯（57.7%），草本精油的主要成分是桧烯（25.9%）。采用肉汤微量稀释法，对这些精油以及之前已报道其成分的奥赫里德岩风地下部分对四种革兰氏阳性菌、三种革兰氏阴性菌和两种白色念珠菌菌株的抗菌活性进行了测试。除宽叶岩风地下部分精油外，其他所研究的精油对金黄色葡萄球菌、表皮葡萄球菌、藤黄微球菌或白色念珠菌均表现出较高的抗菌潜力（最低抑菌浓度为13.0 - 73.0μg/ml），与用作参考化合物的百里酚相当，甚至更高。