平板洗涤试验：一种用于检测出芽酵母丝状生长的简单测试。
The plate-washing assay: a simple test for filamentous growth in budding yeast.
作者信息
Cullen Paul J
机构信息
Department of Biological Sciences, State University of New York at Buffalo, Buffalo, New York 14260.
出版信息
Cold Spring Harb Protoc. 2015 Feb 2;2015(2):168-71. doi: 10.1101/pdb.prot085068.
Abstract
Filamentous growth is a foraging response that occurs in fungal species. It allows fungal pathogens to invade cells and tissues of a host organism. Budding yeast undergoes filamentous growth and can invade semisolid agar plates, penetrating the agar surface. These cells cannot be removed by rinsing with water and form an invasive scar. The plate-washing assay is an easy first test for filamentous growth and is performed at low cost with minimal reagents. The assay is versatile: It can be used as a teaching tool, is amenable to high-throughput genetic analysis, and is used to evaluate filamentous growth in different fungal species, including pathogens like Candida albicans.
摘要
丝状生长是真菌物种中出现的一种觅食反应。它使真菌病原体能够侵入宿主生物体的细胞和组织。出芽酵母会经历丝状生长，并能侵入半固体琼脂平板，穿透琼脂表面。这些细胞不能通过水洗去除，会形成侵入性瘢痕。平板洗涤试验是检测丝状生长的一种简单的初步试验，成本低且所需试剂最少。该试验用途广泛：它可用作教学工具，适用于高通量遗传分析，还可用于评估不同真菌物种的丝状生长，包括白色念珠菌等病原体。
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