从冰岛衣中分离得到的提取物和化合物的体外抗菌活性。
In vitro antimicrobial activity of extracts and compounds isolated from Cladonia uncialis.
作者信息
Studzińska-Sroka Elżbieta, Hołderna-Kędzia Elżbieta, Galanty Agnieszka, Bylka Wiesława, Kacprzak Karol, Ćwiklińska Karolina
机构信息
a Department of Pharmacognosy , Poznan University of Medical Sciences , Święcickiego 4, 60-781 Poznań , Poland.
b Institute of Natural Fibres and Medicinal Plants , Libelta 27, 61-707 Poznań , Poland.
出版信息
Nat Prod Res. 2015;29(24):2302-7. doi: 10.1080/14786419.2015.1005616. Epub 2015 Feb 3.
Heptane (Hep), diethyl ether (Et2O), acetone (Me2CO) and methanolic (MeOH) extracts, as well as (-)-usnic acid and squamatic acid, were obtained from thallus of Cladonia uncialis (Cladoniaceae). The antimicrobial activities of these extracts, (-)-usnic acid and squamatic acid, were tested against reference strains: Staphylococcus aureus, Escherichia coli and Candida albicans. In addition, Me2CO extract was analysed against 10 strains of Methicillin-resistant S. aureus (MRSA) isolated from patients. All extracts exerted antibacterial activity against the reference strain S. aureus, comparably to chloramphenicol [minimum inhibitory concentration (MIC) = 5.0 μg/mL]. The Me2CO extract exhibited the strongest activity against S. aureus (MIC = 0.5 μg/mL), higher than (-)-usnic acid, whereas squamatic acid proved inactive. The Me2CO extract showed potent antimicrobial activity against MRSA (MIC 2.5-7.5 μg/mL). Also no activity of C. uncialis extracts against E. coli and C. albicans was observed.
从石蕊科冰岛石蕊的叶状体中获得了庚烷（Hep）、乙醚（Et2O）、丙酮（Me2CO）和甲醇提取物，以及（-）-松萝酸和鳞片酸。测试了这些提取物、（-）-松萝酸和鳞片酸对参考菌株金黄色葡萄球菌、大肠杆菌和白色念珠菌的抗菌活性。此外，还分析了丙酮提取物对从患者中分离出的10株耐甲氧西林金黄色葡萄球菌（MRSA）的抗菌活性。所有提取物对参考菌株金黄色葡萄球菌均表现出抗菌活性，与氯霉素相当[最低抑菌浓度（MIC）=5.0μg/mL]。丙酮提取物对金黄色葡萄球菌表现出最强的活性（MIC=0.5μg/mL），高于（-）-松萝酸，而鳞片酸则无活性。丙酮提取物对MRSA表现出强大的抗菌活性（MIC 2.5-7.5μg/mL）。此外，未观察到冰岛石蕊提取物对大肠杆菌和白色念珠菌有活性。
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