鲤（芙蓉鲤♀）×兴国红鲤（♂）完整线粒体基因组的遗传
Inheritance of the complete mitochondrial genomes Cyprinus capio furong(♀) × Cyprinus carpio var.singguonensis(♂).
作者信息
Peng Huizhen, Liu Qiaolin, Xiao Tiaoyi
机构信息
a College of Animal Science and Technology, Hunan Agricultural University , Changsha , China.
出版信息
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Sep;27(5):3105-6. doi: 10.3109/19401736.2015.1007286. Epub 2015 Feb 4.
In this study, 15 sets of primers were used to amplify contiguous, overlapping segments of the complete mitochondrial DNA (mtDNA) of C. capio furong(♀) × C. carpio var.singguonensis(♂) in order to characterize and compare their mitochondrial genomes. The total length of the mitochondrial genome was 16,581 bp and deposited in the GenBank with the accession number KP210473. The organization of the mitochondrial genomes contained 37 genes (13 protein-coding genes, 2 ribosomal RNA and 22 transfer RNAs) and a major non-coding control region which was similar to those reported mitochondrial genomes. Most genes were encoded on the H-strand, except for the ND6 and 8 tRNA genes, encoding on the L-strand. The nucleotide skewness for the coding strands of C. capio furong(♀) × C. carpio var.singguonensis(♂) (AT-skew = 0.12, GC-skew = -0.27) were biased toward T and G. The complete mitogenome may provide important date for the study of genetic mechanism of C. capio furong(♀) × C. carpio var.singguonensis(♂).
在本研究中，使用15对引物扩增了芙蓉鲤（♀）×兴国红鲤（♂）完整线粒体DNA（mtDNA）的连续重叠片段，以对其线粒体基因组进行表征和比较。线粒体基因组全长16,581 bp，并以登录号KP210473保存在GenBank中。线粒体基因组的结构包含37个基因（13个蛋白质编码基因、2个核糖体RNA和22个转移RNA）以及一个主要的非编码控制区，这与已报道的线粒体基因组相似。除了在轻链上编码的ND6和8个tRNA基因外，大多数基因在重链上编码。芙蓉鲤（♀）×兴国红鲤（♂）编码链的核苷酸偏度（AT偏度=0.12，GC偏度=-0.27）偏向于T和G。完整的线粒体基因组可能为芙蓉鲤（♀）×兴国红鲤（♂）遗传机制的研究提供重要数据。
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