A proteomics survey on wheat susceptibility to head blight during grain development.

The mycotoxigenic fungal species is able to attack several important cereal crops, such as wheat and barley. By causing Head Blight (FHB) disease, induces yield and quality losses and poses a public health concern due to mycotoxin production. The molecular and physiological plant responses to FHB, and the cellular biochemical pathways used by to complete its infectious process remain still unknown. In this study, a proteomics approach, combining 2D-gel approach and mass spectrometry, has been used to determine the specific protein patterns associated with the development of the fungal infection during grain growth on susceptible wheat. Our results reveal that infection does not deeply alter the grain proteome and does not significantly disturb the first steps of grain ontogeny but impacts molecular changes during the grain filling stage (impact on starch synthesis and storage proteins). The differentially regulated proteins identified were mainly involved in stress and defence mechanisms, primary metabolism, and main cellular processes such as signalling and transport. Our survey suggests that could take advantage of putative susceptibility factors closely related to grain development processes and thus provide new insights into key molecular events controlling the susceptible response to FHB in wheat grains.