免疫相关血细胞减少症中发生改变的IgG自身抗体亚类

IgG autoantibody subclasses altered in immuno-related hemocytopenia.

作者信息

Shao Yuanyuan, Fu Rong, Liu Hui, Wang Yihao, Ding Shaoxue, Wang Huaquan, Li Lijuan, Shao Zonghong

机构信息

Department of Hematology, Tianjin Medical University General Hospital, 154 Anshan St, Heping District, Tianjin 300052, PR China.

出版信息

Cell Immunol. 2015 Mar;294(1):13-20. doi: 10.1016/j.cellimm.2015.01.013. Epub 2015 Jan 31.

DOI:10.1016/j.cellimm.2015.01.013

PMID:25666506

Abstract

Recently, we have detected autoantibodies to bone marrow (BM) hemopoietic cells in some idiopathic cytopenia of undetermined significance (ICUS), terming as immunorelated hemocytopenia. Immunoglobulin G consists of 4 subclasses which have different biological functions in many diseases. In this study, we analyzed the alterations of IgG subclasses in 27 IRH patients compared with 20 ICUS patients and 20 normal controls. The results showed that IgG1 and IgG3 levels were increased in the bone marrow supernatant in IRH patients, and had inverse correlations with hematopoietic function. Meanwhile IgG1 level had a positive correlation with the proportion of CD5+ B lymphocytes. Using immunohistochemical staining, IgG1 were also detected on bone marrow nucleated cells in IRH patients. All these results indicated that IgG1 on bone marrow cells in some IRH patients might be involved in the destruction of hematopoietic cells leading to cytopenia and might be a novel therapeutic target in future.

摘要

最近，我们在一些意义未明的特发性血细胞减少症（ICUS）中检测到了针对骨髓（BM）造血细胞的自身抗体，将其称为免疫相关性血细胞减少症。免疫球蛋白G由4个亚类组成，在许多疾病中具有不同的生物学功能。在本研究中，我们分析了27例免疫相关性血细胞减少症（IRH）患者与20例ICUS患者及20例正常对照者的IgG亚类变化。结果显示，IRH患者骨髓上清液中IgG1和IgG3水平升高，且与造血功能呈负相关。同时，IgG1水平与CD5+B淋巴细胞比例呈正相关。采用免疫组织化学染色法，在IRH患者的骨髓有核细胞上也检测到了IgG1。所有这些结果表明，部分IRH患者骨髓细胞上的IgG1可能参与了造血细胞的破坏，导致血细胞减少，可能是未来新的治疗靶点。