通过刃天青肉汤微量稀释法检测快速生长分枝杆菌的药物敏感性。
Detection of drug susceptibility in rapidly growing mycobacteria by resazurin broth microdilution assay.
作者信息
Castilho Aline Lemes, Caleffi-Ferracioli Katiany Rizzieri, Canezin Pedro Henrique, Dias Siqueira Vera Lúcia, de Lima Scodro Regiane Bertin, Cardoso Rosilene Fressatti
机构信息
Postgraduate Program in Health Science, State University of Maringá, Paraná, Brazil.
Laboratory of Medical Bacteriology, Department of Clinical Analysis and Biomedicine, State University of Maringá, Paraná, Brazil.
出版信息
J Microbiol Methods. 2015 Apr;111:119-21. doi: 10.1016/j.mimet.2015.02.007. Epub 2015 Feb 12.
The susceptibility of rapidly growing mycobacteria (RGM) to linezolid and ciprofloxacin was evaluated by using resazurin as a growth indicator. The assay with resazurin supplemented medium performed as well as its addition to the medium at reading time and was efficient for the determination for the susceptibility profile in RGM.
通过使用刃天青作为生长指示剂，评估快速生长分枝杆菌（RGM）对利奈唑胺和环丙沙星的敏感性。含有刃天青补充培养基的检测方法与在读取结果时将其添加到培养基中的方法效果相同，并且对于确定RGM的敏感性谱是有效的。
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