耻垢分枝杆菌MSMEG_3705编码一种具有多种功能的选择性主要易化子超家族外排泵。
Mycobacterium smegmatis MSMEG_3705 encodes a selective major facilitator superfamily efflux pump with multiple roles.
作者信息
Zhang Zhen, Wang Rui, Xie Jianping
机构信息
Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Three Gorges Eco-environment and Bioresources, Key Laboratory Ministry of Education Eco-Environment of the Three Gorges Reservoir Region, School of Life Sciences, Southwest University, Chongqing, 400715, China,
出版信息
Curr Microbiol. 2015 Jun;70(6):801-9. doi: 10.1007/s00284-015-0783-0. Epub 2015 Feb 20.
Mycobacterium smegmatis mc(2)155 MSMEG_3705 gene was annotated to encode a transporter protein that contains 12 alpha-helical transmembrane domains. We predicted MSMEG_3705 encoding a major facilitator superfamily (MFS) member. To confirm the prediction, the M. smegmatis mc(2)155 MSMEG_3705 gene was deleted. The MSMEG_3705 deletion mutant strain M. smegmatis mc(2)155 ∆MSMEG_3705 was more sensitive to capreomycin. Moreover, M. smegmatis mc(2)155 ∆MSMEG_3705 strain accumulated more ethidium bromide intracellular than wild-type M. smegmatis mc(2)155. Quite unexpectedly, M. smegmatis mc(2)155 ∆MSMEG_3705 grew faster than the wild-type M. smegmatis mc(2)155. The upregulation of the expression of MSMEG_3706, a gene encoding isocitrate lyase downstream MSMEG_3705, in the deletion mutant, might underlie such faster growth in the mutant. The study showed that MSMEG_3705 encodes a genuine MFS member and plays significant role in bacterial growth and antibiotics resistance.
耻垢分枝杆菌mc(2)155的MSMEG_3705基因被注释为编码一种含有12个α-螺旋跨膜结构域的转运蛋白。我们预测MSMEG_3705编码一个主要易化子超家族（MFS）成员。为了证实这一预测，删除了耻垢分枝杆菌mc(2)155的MSMEG_3705基因。MSMEG_3705缺失突变株耻垢分枝杆菌mc(2)155 ∆MSMEG_3705对卷曲霉素更敏感。此外，耻垢分枝杆菌mc(2)155 ∆MSMEG_3705菌株细胞内积累的溴化乙锭比野生型耻垢分枝杆菌mc(2)155更多。非常出乎意料的是，耻垢分枝杆菌mc(2)155 ∆MSMEG_3705比野生型耻垢分枝杆菌mc(2)155生长得更快。在缺失突变体中，MSMEG_3705下游编码异柠檬酸裂合酶的基因MSMEG_3706表达上调，可能是该突变体生长更快的原因。该研究表明，MSMEG_3705编码一个真正的MFS成员，并且在细菌生长和抗生素抗性中发挥重要作用。
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