Zhang Yue, Campbell Rachel, Drake MaryAnne, Zhong Qixin
Department of Food Science and Technology, University of Tennessee, Knoxville 37996.
Southeast Dairy Foods Research Center, Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh 27695.
J Dairy Sci. 2015 May;98(5):2982-91. doi: 10.3168/jds.2014-9159. Epub 2015 Feb 20.
Colored Cheddar whey is a source for whey protein recovery and is decolorized conventionally by bleaching, which affects whey protein quality. Two activated carbons were studied in the present work as physical means of removing annatto (norbixin) in Cheddar cheese whey. The color and residual norbixin content of Cheddar whey were reduced by a higher level of activated carbon at a higher temperature between 25 and 55°C and a longer time. Activated carbon applied at 40g/L for 2h at 30°C was more effective than bleaching by 500mg/L of hydrogen peroxide at 68°C. The lowered temperature in activated-carbon treatments had less effect on protein structure as investigated for fluorescence spectroscopy and volatile compounds, particularly oxidation products, based on gas chromatography-mass spectrometry. Activated carbon was also reusable, removing more than 50% norbixin even after 10 times of regeneration, which showed great potential for decolorizing cheese whey.
有色切达干酪乳清是乳清蛋白回收的一个来源,传统上通过漂白进行脱色,这会影响乳清蛋白的质量。在本研究中,研究了两种活性炭作为去除切达干酪乳清中胭脂树橙(降胭脂树素)的物理方法。在25至55°C的较高温度下,使用较高水平的活性炭并延长时间,可以降低切达干酪乳清的颜色和降胭脂树素残留量。在30°C下以40g/L的活性炭处理2小时比在68°C下用500mg/L的过氧化氢漂白更有效。根据荧光光谱和基于气相色谱-质谱联用仪的挥发性化合物(特别是氧化产物)研究,活性炭处理中较低的温度对蛋白质结构的影响较小。活性炭还可重复使用,即使经过10次再生后仍能去除超过50%的降胭脂树素,这表明其在奶酪乳清脱色方面具有巨大潜力。