Topel Seda Demirel, Topel Önder, Bostancıoğlu R Beklem, Koparal A Tansu
Department of Chemistry, Faculty of Science, Akdeniz University, 07058 Antalya, Turkey.
Department of Biology, Faculty of Science, Anadolu University, Eskişehir, Turkey.
Colloids Surf B Biointerfaces. 2015 Apr 1;128:245-253. doi: 10.1016/j.colsurfb.2015.01.043. Epub 2015 Feb 7.
Multifunctional magnetic nanoparticles were synthesized for potential bio-imaging applications. Uniform PEI coated magnetic Fe3O4 (PEI-Fe3O4) nanoparticles were prepared by a modified co-precipitation method and then covalently conjugated with a fluorophore molecule, Bodipy-5 by the DCC/DMAP coupling reaction. The covalent binding of Bodipy-5 to the PEI coated magnetic Fe3O4 nanoparticles were confirmed by means of FTIR and XPS measurements. The imaging ability of the Bodipy coated magnetic nanoparticles was determined on two human cancer cells, A549 (human lung adenocarcinoma epithelial) and Ishikawa (endometrial adenocarcinoma), for the first time. Cytotoxicity of BOD-MNPs was evaluated in both cancer cells and healthy human umbilical vein endothelial cell line (HUVEC) by standard MTT (3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay. In vitro activities of the nanoparticles were also investigated.
合成了多功能磁性纳米颗粒用于潜在的生物成像应用。通过改进的共沉淀法制备了均匀的聚乙二醇包覆的磁性Fe3O4（PEI-Fe3O4）纳米颗粒，然后通过DCC/DMAP偶联反应将其与荧光团分子Bodipy-5共价结合。通过FTIR和XPS测量证实了Bodipy-5与聚乙二醇包覆的磁性Fe3O4纳米颗粒的共价结合。首次在两种人类癌细胞A549（人肺腺癌上皮细胞）和Ishikawa（子宫内膜腺癌）上测定了Bodipy包覆的磁性纳米颗粒的成像能力。通过标准的MTT（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑）法评估了BOD-MNPs在癌细胞和健康人脐静脉内皮细胞系（HUVEC）中的细胞毒性。还研究了纳米颗粒的体外活性。
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