猴胰腺中免疫反应性生长抑素的含量与组成

The content and composition of immunoreactive somatostatin in monkey pancreas.

作者信息

Yeo T M, Liu Y F, Kou M T, Perng J C, Ho L T

出版信息

Zhonghua Yi Xue Za Zhi (Taipei). 1989 Apr;43(4):237-42.

Abstract

A highly sensitive and specific radioimmunoassay (RIA) was developed for analyzing somatostatin (SRIF-LI). The monkey pancreas was lyophilized and extracted with 2N acetic acid. The content and composition of immunoreactive somatostatin in monkey pancreas were then evaluated by chromatography process and RIA. The concentration of SRIF-LI in monkey pancreas was around 513.8 ng/g dry weight. At least 3 components of SRIF-LI were detected in pancreatic extracts. The major component of SRIF-LI (about 92%) was SS-14, and other two were SS-28 and a form bigger than SS-28.

摘要

为分析生长抑素（SRIF-LI），开发了一种高灵敏度和特异性的放射免疫测定法（RIA）。将猴胰腺冻干并用2N乙酸提取。然后通过色谱法和放射免疫测定法评估猴胰腺中免疫反应性生长抑素的含量和组成。猴胰腺中SRIF-LI的浓度约为513.8 ng/g干重。在胰腺提取物中检测到至少3种SRIF-LI成分。SRIF-LI的主要成分（约92%）是SS-14，另外两种是SS-28和一种比SS-28更大的形式。