溶藻弧菌谷氨酰胺合成酶基因区域的核苷酸序列。

Nucleotide sequence of the Vibrio alginolyticus glnA region.

作者信息

Maharaj R, Rumbak E, Jones W A, Robb S M, Robb F T, Woods D R

机构信息

Department of Microbiology, University of Cape Town, Rondebosch, South Africa.

出版信息

Arch Microbiol. 1989;152(6):542-9. doi: 10.1007/BF00425484.

DOI:10.1007/BF00425484

PMID:2574025

Abstract

The nucleotide sequence of a 4 kb fragment containing the Vibrio alginolyticus glnA, ntrB and ntrC genes was determined. The upstream region of the glnA gene contained tandem promoters. The upstream promoter resembled the consensus sequence for Escherichia coli sigma 70 promoters whereas the presumptive downstream promoter showed homology with nitrogen regulated promoters. Four putative NRI binding sites were located between the tandem promoters. The ntrB gene was preceded by a single presumptive NRI binding site. The ntrC gene was located 45 base pairs downstream from the ntrB gene. The V. alginolyticus ntrB and ntrC genes were able to complement ntrB, ntrC deletions in E. coli.

摘要

测定了包含溶藻弧菌谷氨酰胺合成酶基因（glnA）、氮调节蛋白B基因（ntrB）和氮调节蛋白C基因（ntrC）的一个4kb片段的核苷酸序列。glnA基因的上游区域含有串联启动子。上游启动子类似于大肠杆菌σ70启动子的共有序列，而推测的下游启动子与氮调节启动子具有同源性。在串联启动子之间有四个假定的NRI结合位点。ntrB基因之前有一个单一的假定NRI结合位点。ntrC基因位于ntrB基因下游45个碱基对处。溶藻弧菌的ntrB和ntrC基因能够互补大肠杆菌中ntrB、ntrC的缺失。