一项关于基于硫酸钙的骨移植材料与人牙髓干细胞（hDPSCs）异种移植的体内猪研究。
An in vivo swine study for xeno-grafts of calcium sulfate-based bone grafts with human dental pulp stem cells (hDPSCs).
作者信息
Kuo Tzong-fu, Lee Sheng-Yang, Wu Hong-Da, Poma Malosi, Wu Yu-Wei, Yang Jen-Chang
机构信息
Department of Veterinary Medicine, National Taiwan University, Taipei 110, Taiwan, ROC; Center for Teeth Bank and Dental Stem Cell Technology, Taipei Medical University, Taipei 110, Taiwan, ROC.
School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei 110, Taiwan, ROC; Dental Department, Wan-Fang Hospital, Taipei Medical University, Taipei 110, Taiwan, ROC; Center for Teeth Bank and Dental Stem Cell Technology, Taipei Medical University, Taipei 110, Taiwan, ROC.
出版信息
Mater Sci Eng C Mater Biol Appl. 2015 May;50:19-23. doi: 10.1016/j.msec.2015.01.092. Epub 2015 Jan 31.
The purpose of this in vivo study was to evaluate the effect of human dental pulp stem cells (hDPSCs) on various resorbable calcium sulfate/calcium phosphate bone grafts in bone regeneration. Granular particles of calcium sulfate dehydrate (CSD), α-calcium sulfate hemihydrate/amorphous calcium phosphate (α-CSH/ACP), and CSD/β-tricalcium phosphates (β-TCP) were prepared for in vitro dissolution and implantation test. The chemical compositions of specimen residues after dissolution test were characterized by XRD. The ratios of new bone formation for implanted grafts/hDPSCs were evaluated using mandible bony defect model of Lanyu pig. All the graft systems exhibited a similar two-stage dissolution behavior and phase transformation of poor crystalline HAp. Eight weeks post-operation, the addition of hDPSCs to various graft systems showed statistically significant increasing in the ratio of new bone formation (p<0.05). Null hypothesis of hDPSCs showing no scaffold dependence in bone regeneration was rejected. The results suggest that the addition of hDPSCs to calcium sulfate based xenografts could enhance the bone regeneration in the bony defect.
本体内研究的目的是评估人牙髓干细胞（hDPSCs）对各种可吸收硫酸钙/磷酸钙骨移植材料在骨再生中的作用。制备了硫酸钙脱水物（CSD）、α-半水硫酸钙/无定形磷酸钙（α-CSH/ACP）和CSD/β-磷酸三钙（β-TCP）的颗粒用于体外溶解和植入试验。通过XRD对溶解试验后标本残余物的化学成分进行表征。使用蓝玉猪下颌骨骨缺损模型评估植入移植物/hDPSCs的新骨形成比例。所有移植物系统均表现出类似的两阶段溶解行为和低结晶度HAp的相变。术后8周，在各种移植物系统中添加hDPSCs显示新骨形成比例有统计学意义的增加（p<0.05）。hDPSCs在骨再生中不显示支架依赖性的原假设被拒绝。结果表明，在基于硫酸钙的异种移植物中添加hDPSCs可增强骨缺损处的骨再生。
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