考虑到用于采集脂肪组织的设备方法，负压对脂肪细胞和间充质干细胞活力的影响。
Influence of negative pressure on the viability of adipocytes and mesenchymal stem cell, considering the device method used to harvest fat tissue.
作者信息
Charles-de-Sá Luiz, Gontijo de Amorim Natale F, Dantas Danielle, Han Joh Victor, Amable Paola, Teixeira Marcus Vinicius Telles, de Araújo Pedro Luiz, Link Walter, Borojevich Radovan, Rigotti Gino
机构信息
Dr Charles-de-Sá is an Associate Professor, Department of Plastic, Reconstructive, and Aesthetic Surgery, Training and Research State University Hospital, Rio de Janeiro, UERJ, Brazil. Dr de Amorim is a Professor of Plastic Surgery, Pontifical Catholic University - PUC Rio and Carlos Chagas Post-graduation Institute; and a Researcher and Professor Coordinator of the Scientific Department, the Ivo Pitanguy Institute, Rio de Janiero, Brazil. Dr Dantas is a plastic surgeon in private practice, Rio de Janeiro, Brazil. Dr Han is a general surgeon in private practice in Natal-RN, Brazil. Drs Amable and Teixeira are Biologists, Excellion Biomedical Services, Petrópolis, RJ, Brazil. Drs de Araújo and Link are Engineers and Professors, The Center for Technology, Department of Mechanical Engineering, Laboratory of Metrology, Rio Grande do Norte Federal University (UFRN), Brazil. Dr Borojevich is a Biologist, Senior Researcher, and Professor, The Institute of Biomedical Sciences, Federal University of Rio de Janeiro, RJ, Brazil. Dr Rigotti is a plastic surgeon in private practice in Verona, Italy.
出版信息
Aesthet Surg J. 2015 Mar;35(3):334-44. doi: 10.1093/asj/sju047.
BACKGROUND
Transplanted adipose tissue has many applications in regenerative medicine. However, fat grafting yields unpredictable results because the fat that is transferred can suffer variable degrees of fat reabsorption. It is necessary to identify methods and maneuvers to minimize reabsorption rates and provide predictable long-term results.
OBJECTIVES
Our study aimed to identify the optimal method of harvesting, as well as the optimal pressure regime for fat aspiration. The primary objective was to assess the degree of adipocyte and mesenchymal stem cell death that occurred with the various devices and pressure levels used to harvest fat.
METHODS
This study was a prospective, randomized, comparative study in 15 healthy male and female subjects aged 25 to 60 who were undergoing abdominal cosmetic surgery. Various apparatuses and pressure regimens were used to harvest 8 samples of fat tissue. These samples (R1 = R8) underwent histological analysis in order to verify the integrity and functionality of the adipocytes and mesenchymal stem cells that had been harvested.
RESULTS
A total of 14 females and 1 male underwent abdominal cosmetic surgery. Quantitative analysis revealed that the adipocytes in all 8 samples had homogeneous quantitative profiles. The adipose mesenchymal stem cell (AMSC) analysis, according to Friedman ANOVA, revealed no significant variation in the percentage of mesenchymal stem cells (P = .045) between the various samples.
CONCLUSIONS
The type of device, nozzle diameter tip, and pressure regimen used in this study for harvesting fat tissue did not significantly affect the number of the adipocytes or viable AMSC harvested.
LEVEL OF EVIDENCE
3 Therapeutic.
背景
移植的脂肪组织在再生医学中有许多应用。然而，脂肪移植的效果不可预测，因为移植的脂肪会发生不同程度的再吸收。有必要确定方法和操作以尽量减少再吸收率并提供可预测的长期效果。
目的
我们的研究旨在确定收获脂肪的最佳方法以及脂肪抽吸的最佳压力方案。主要目的是评估在用于收获脂肪的各种设备和压力水平下发生的脂肪细胞和间充质干细胞死亡程度。
方法
本研究是一项前瞻性、随机、对比研究，纳入了15名年龄在25至60岁之间接受腹部整形手术的健康男性和女性受试者。使用各种设备和压力方案收获8份脂肪组织样本。这些样本（R1 = R8）进行了组织学分析，以验证所收获的脂肪细胞和间充质干细胞的完整性和功能。
结果
共有14名女性和1名男性接受了腹部整形手术。定量分析显示，所有8个样本中的脂肪细胞具有均匀的定量特征。根据弗里德曼方差分析，脂肪间充质干细胞（AMSC）分析显示，不同样本之间的间充质干细胞百分比无显著差异（P = 0.045）。
结论
本研究中用于收获脂肪组织的设备类型、喷嘴直径尖端和压力方案对所收获的脂肪细胞数量或存活的AMSC数量没有显著影响。
证据水平
3级治疗性。
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