Çakal Arslan Özlem, Boyacioğlu Meltem, Parlak Hatice, Katalay Selma, Karaaslan Muhammet Ali
Faculty of Fisheries, Ege University, 35100 Bornova, Izmir, Turkey.
Faculty of Fisheries, Ege University, 35100 Bornova, Izmir, Turkey.
Mar Pollut Bull. 2015 May 15;94(1-2):48-54. doi: 10.1016/j.marpolbul.2015.03.018. Epub 2015 Mar 21.
The aim of this paper was to assess the biological damages in fish caused by various mutagenic agents present in polluted waters of Aliağa Bay. For this purpose, micronuclei (MN) test was performed using peripheral erythrocytes and gill cells of different fish specimens caught from both polluted and relatively clean sites from Aliağa Bay (Turkey). Micronuclei tests is a system of mutagenicity testing used for determining changes in DNA fragments such as micronuclei in the cytoplasm of interphase cells caused by the pollution and chemicals in the environment. Thus, it was attempted to determine whether pollution affected the erythrocytes and gills of fish living in Aliağa Bay at the level of DNA by the means of micronuclei (MN) test. According to the results of present study, frequency of MN was found at high level in polluted site. In conclusion, this study indicates that the micronuclei test gives sensitive results in monitoring the pollution, especially the pollution of harbor, and thus it might be used as standard method in regularly monitoring pollution of coastal ecosystem.
本文的目的是评估阿利亚加湾污染水域中存在的各种诱变剂对鱼类造成的生物损害。为此,使用从土耳其阿利亚加湾污染和相对清洁地点捕获的不同鱼类样本的外周血红细胞和鳃细胞进行了微核(MN)试验。微核试验是一种用于确定环境中的污染和化学物质导致的间期细胞胞质中微核等DNA片段变化的致突变性检测系统。因此,试图通过微核(MN)试验确定污染是否在DNA水平上影响了生活在阿利亚加湾的鱼类的红细胞和鳃。根据本研究结果,在污染地点发现微核频率处于高水平。总之,本研究表明微核试验在监测污染,特别是港口污染方面给出了敏感结果,因此它可能被用作定期监测沿海生态系统污染的标准方法。