Nanog 的多能性活性需要变体组蛋白 H2A.Z 的生化稳定化。
Pluripotency Activity of Nanog Requires Biochemical Stabilization by Variant Histone Protein H2A.Z.
机构信息
CAS Key Laboratory of Innate Immunity and Chronic Disease, Innovation Center for Cell Signaling Network, School of Life Sciences and Medical Center, University of Science & Technology of China, Hefei, People's Republic of China.
Stem Cell and Regenerative Biology, Genome Institute of Singapore, Singapore, Singapore.
出版信息
Stem Cells. 2015 Jul;33(7):2126-34. doi: 10.1002/stem.2011. Epub 2015 May 21.
The variant histone protein H2A.Z plays a critical role in early development. Likewise, Nanog, a master regulator of embryonic stem cells (ESCs), is essential for proper development in early embryogenesis. In this study, we establish that these two factors work together to maintain pluripotency. It is shown that H2A.Z influences the protein level of Nanog through the ubiquitin-proteasome pathway. Knockdown of H2A.Z causes differentiation of mouse ESCs and disrupts the reprogramming of somatic cells, which can be partially rescued by overexpression of Nanog. We conclude that the H2A.Z-Nanog partnership is involved in ESC pluripotency and reprogramming of somatic cells. Stem Cells 2015;33:2126-2134.
变体组蛋白 H2A.Z 在早期发育中起着关键作用。同样，胚胎干细胞 (ESC) 的主调控因子 Nanog 对于早期胚胎发生中的正常发育也是必不可少的。在这项研究中，我们确定这两个因素共同作用以维持多能性。结果表明，H2A.Z 通过泛素蛋白酶体途径影响 Nanog 的蛋白水平。H2A.Z 的敲低导致小鼠 ESC 的分化，并破坏体细胞的重编程，而过表达 Nanog 可部分挽救这种现象。我们的结论是，H2A.Z-Nanog 伙伴关系参与了 ESC 的多能性和体细胞的重编程。《干细胞》 2015 年；33:2126-2134。
Zotero 插件