G蛋白信号转导激活因子3的过表达降低食管鳞状细胞癌的增殖。
Overexpression of activator of G-protein signaling 3 decreases the proliferation of esophageal squamous cell carcinoma.
作者信息
Shi Hui, Ren Hanru, Yang Xiaojing, Zhu Hongzhen, Yao Li, Hang Qinglei, Mao Hui, Huang Yuejiao, Zhang Jianguo, Wang Yuchan
机构信息
Department of Thoracic Surgery, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu, PR China.
Department of Thoracic Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai 200120, PR China.
出版信息
Pathol Res Pract. 2015 Jun;211(6):449-55. doi: 10.1016/j.prp.2014.12.016. Epub 2015 Feb 19.
BACKGROUND
Activator of G-protein Signaling 3 (AGS3, also known as GPSM1), is related to cell cycle progression. We investigated the expression of AGS3 in human esophageal squamous cell carcinoma (ESCC) and the therapeutic effect of chemotherapy drugs.
METHODS
Immunohistochemistry and Western blot analysis were performed for AGS3 in 85ESCC samples. The data were correlated with clinicopathological features. The univariate and multivariate survival analyses were also performed to determine its prognostic significance. The effect of overexpression of AGS3 on proliferation of esophageal carcinoma TE1 cells was analyzed by serum starvation.
RESULTS
AGS3 was down regulated in ESCC as compared with the adjacent normal tissue. Low expression of AGS3 was associated with tumor grade (P=0.002), and AGS3 was negatively correlated with proliferation marker Ki-67 (P<0.01). Univariate analysis showed that AGS3 expression did have a remarkable prediction for poor prognosis (P=0.004), while in vitro, the expression of AGS3 was down regulated with release from serum starvation of TE1 cells.
CONCLUSIONS
This study shows that AGS3 is an important regulator of ESCC proliferation.
背景
G蛋白信号转导激活因子3（AGS3，也称为GPSM1）与细胞周期进程相关。我们研究了AGS3在人食管鳞状细胞癌（ESCC）中的表达及化疗药物的治疗效果。
方法
对85例ESCC样本进行AGS3的免疫组织化学和蛋白质印迹分析。将数据与临床病理特征相关联。还进行了单因素和多因素生存分析以确定其预后意义。通过血清饥饿分析AGS3过表达对食管癌TE1细胞增殖的影响。
结果
与相邻正常组织相比，ESCC中AGS3表达下调。AGS3低表达与肿瘤分级相关（P = 0.002），且AGS3与增殖标志物Ki-67呈负相关（P <0.01）。单因素分析显示AGS3表达对预后不良具有显著预测作用（P = 0.004），而在体外，随着TE1细胞血清饥饿解除，AGS3表达下调。
结论
本研究表明AGS3是ESCC增殖的重要调节因子。
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