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一种用于检测传染源的移动式生物安全微量分析系统。

A mobile biosafety microanalysis system for infectious agents.

作者信息

Beniac Daniel R, Hiebert Shannon L, Siemens Christine G, Corbett Cindi R, Booth Tim F

机构信息

National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba. R3E 3R2, Canada.

1] National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba. R3E 3R2, Canada [2] Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba. R3E 0W3, Canada.

出版信息

Sci Rep. 2015 Mar 30;5:9505. doi: 10.1038/srep09505.

DOI:10.1038/srep09505
PMID:25820944
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4377622/
Abstract

Biological threats posed by pathogens such as Ebola virus must be quickly diagnosed, while protecting the safety of personnel. Scanning electron microscopy and microanalysis requires minimal specimen preparation and can help to identify hazardous agents or substances. Here we report a compact biosafety system for rapid imaging and elemental analysis of specimens, including powders, viruses and bacteria, which is easily transportable to the site of an incident.

摘要

必须在保护人员安全的同时,快速诊断埃博拉病毒等病原体构成的生物威胁。扫描电子显微镜和微量分析所需的样本制备极少,有助于识别危险因子或物质。在此,我们报告一种紧凑的生物安全系统,用于对包括粉末、病毒和细菌在内的样本进行快速成像和元素分析,该系统易于运输到事故现场。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cf1/4377622/f72e90991def/srep09505-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cf1/4377622/f688672e1914/srep09505-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cf1/4377622/f72e90991def/srep09505-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cf1/4377622/f688672e1914/srep09505-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8cf1/4377622/f72e90991def/srep09505-f2.jpg

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1
A mobile biosafety microanalysis system for infectious agents.一种用于检测传染源的移动式生物安全微量分析系统。
Sci Rep. 2015 Mar 30;5:9505. doi: 10.1038/srep09505.
2
The scanning electron microscope in microbiology and diagnosis of infectious disease.扫描电子显微镜在微生物学和传染病诊断中的应用。
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3
Infection at the cellular level.细胞层面的感染。
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4
X-ray microanalysis in the scanning electron microscope.扫描电子显微镜中的X射线微分析
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X-ray microanalysis with the environmental scanning electron microscope: interpretation of data obtained under different atmospheric conditions.环境扫描电子显微镜的X射线微分析:不同大气条件下所获数据的解读
Scanning Microsc Suppl. 1994;8:219-27; discussion 227-9.
6
Preparation of microbiological specimens for scanning electron microscopy.用于扫描电子显微镜检查的微生物标本制备
Scan Electron Microsc. 1980(Pt 2):45-56.
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Application of SEM and EDX in studying biomineralization in plant tissues.扫描电子显微镜(SEM)和能量散射X射线光谱(EDX)在植物组织生物矿化研究中的应用。
Methods Mol Biol. 2014;1117:663-75. doi: 10.1007/978-1-62703-776-1_29.
8
Elemental microanalysis of biological specimens.生物标本的元素微量分析。
Scanning Microsc. 1988 Jun;2(2):937-46.
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Diagnostic applications of scanning electron microscopy and microanalysis in pathology.扫描电子显微镜及微量分析在病理学中的诊断应用
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Scan Electron Microsc. 1982(Pt 4):1603-15.

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本文引用的文献

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A filtration based technique for simultaneous SEM and TEM sample preparation for the rapid detection of pathogens.一种基于过滤的技术,用于同时制备扫描电子显微镜(SEM)和透射电子显微镜(TEM)样本,以快速检测病原体。
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How do filovirus filaments bend without breaking?丝状病毒丝如何在不断裂的情况下弯曲?
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Rapid and reliable detection of bacterial endospores in environmental samples by diagnostic electron microscopy combined with X-ray microanalysis.
透射电子显微镜提高了对严重肺炎非细菌性病因的诊断敏感性:一项回顾性研究。
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The scanning electron microscope in microbiology and diagnosis of infectious disease.扫描电子显微镜在微生物学和传染病诊断中的应用。
Sci Rep. 2016 May 23;6:26516. doi: 10.1038/srep26516.
利用诊断电子显微镜结合 X 射线微分析快速可靠地检测环境样品中的细菌芽孢。
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Biosafety Level-4 laboratories in Europe: opportunities for public health, diagnostics, and research.欧洲的生物安全4级实验室:公共卫生、诊断和研究的机遇
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Flexibility of mobile laboratory unit in support of patient management during the 2007 Ebola-Zaire outbreak in the Democratic Republic of Congo.移动实验室单元的灵活性在支持 2007 年刚果民主共和国埃博拉-扎伊尔疫情期间的患者管理中的作用。
Zoonoses Public Health. 2012 Sep;59 Suppl 2:151-7. doi: 10.1111/j.1863-2378.2012.01477.x.
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The organisation of Ebola virus reveals a capacity for extensive, modular polyploidy.埃博拉病毒的组织方式揭示了其具有广泛的、模块化的多倍体能力。
PLoS One. 2012;7(1):e29608. doi: 10.1371/journal.pone.0029608. Epub 2012 Jan 11.
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Comparison of quantitative PCR and culture-based methods for evaluating dispersal of Bacillus thuringiensis endospores at a bioterrorism hoax crime scene.定量 PCR 和基于培养的方法评估生物恐怖主义恶作剧犯罪现场苏云金芽孢杆菌内孢子散布的比较。
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The use of a mobile laboratory unit in support of patient management and epidemiological surveillance during the 2005 Marburg Outbreak in Angola.在 2005 年安哥拉马尔堡疫情期间,使用移动实验室单位支持患者管理和流行病学监测。
PLoS Negl Trop Dis. 2011 May;5(5):e1183. doi: 10.1371/journal.pntd.0001183. Epub 2011 May 24.
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Modern uses of electron microscopy for detection of viruses.电子显微镜在病毒检测中的现代应用。
Clin Microbiol Rev. 2009 Oct;22(4):552-63. doi: 10.1128/CMR.00027-09.
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Medical countermeasures to protect humans from anthrax bioterrorism.医学对策保护人类免受炭疽生物恐怖袭击。
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