Chernyshev A I, Davletova Sh K, Bashkirov V I, Shakhmanov N B, Mekhedov S L, Anan'ev E V
Genetika. 1989 Aug;25(8):1349-55.
The gene encoding B1 hordein of Hordeum vulgare (cv. Donetsky 4) was cloned and entirely sequenced. It contains no introns and codes for of 293 amino acids long polypeptide with molecular weight 33418. Our clone differs from the previously sequenced B1 hordein genes in some positions within the coding region (there are 4 nucleotide changes and a 12 bp deletion, as compared with the pB11 cDNA clone, and 5 nucleotide changes, as compared with the pBHP184 genomic clone). These changes result in polymorphism of amino acid sequences at 5 positions. 5'-flanking region contains putative regulatory and promoter sequences and differs from that of the pBHP184 clone in 3 positions.
对大麦（品种Donetsky 4）编码B1醇溶蛋白的基因进行了克隆和全序列测定。该基因不含内含子，编码一个由293个氨基酸组成的多肽，分子量为33418。我们克隆的基因在编码区内的某些位置与先前测序的B1醇溶蛋白基因不同（与pB11 cDNA克隆相比，有4个核苷酸变化和一个12 bp的缺失；与pBHP184基因组克隆相比，有5个核苷酸变化）。这些变化导致在5个位置上氨基酸序列的多态性。5'侧翼区包含推定的调控和启动子序列，在3个位置上与pBHP184克隆不同。
