利用遗传和基因组学方法鉴定莱茵衣藻鞭毛组装相关基因。
Genetic and genomic approaches to identify genes involved in flagellar assembly in Chlamydomonas reinhardtii.
作者信息
Lin Huawen, Dutcher Susan K
机构信息
Department of Genetics, Washington University, St. Louis, MO, USA.
Department of Genetics, Washington University, St. Louis, MO, USA.
出版信息
Methods Cell Biol. 2015;127:349-86. doi: 10.1016/bs.mcb.2014.12.001. Epub 2015 Feb 14.
Abstract
Flagellar assembly requires intraflagellar transport of components from the cell body to the flagellar tip for assembly. The understanding of flagellar assembly has been aided by the ease of biochemistry and the availability of mutants in the unicellular green alga, Chlamydomonas reinhardtii. In this chapter, we discuss means to identify genes involved in these processes using forward and reverse genetics. In particular, the ease and low cost of whole genome sequencing (WGS) will help to make gene identification easier and promote the understanding of this important process.
摘要
鞭毛组装需要将组件从细胞体向鞭毛尖端进行鞭毛内运输以进行组装。单细胞绿藻莱茵衣藻中生物化学操作的简便性以及突变体的可得性有助于对鞭毛组装的理解。在本章中，我们讨论使用正向和反向遗传学来鉴定参与这些过程的基因的方法。特别是，全基因组测序（WGS）的简便性和低成本将有助于更轻松地鉴定基因，并促进对这一重要过程的理解。
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