Liu Shuzhen, Wang Xiaofeng, Shi Yuanyuan, Han Lu, Zhao Zhenzhen, Zhao Chengquan, Luo Bing
Department of Medical Microbiology, Qingdao University Medical College, 38 Dengzhou Road, Qingdao, 266021, China.
Saudi J Gastroenterol. 2015 Mar-Apr;21(2):95-103. doi: 10.4103/1319-3767.153832.
BACKGROUND/AIMS: Various polymorphisms in toll-like receptor (TLR) genes have been identified and associated with susceptibility to various malignancies, such as gastric carcinoma (GC), breast cancer, and prostate cancer. However, little is known about the polymorphisms of TLR genes and the susceptibility to GC in Northern China, especially to Epstein-Barr virus-associated GC (EBVaGC). We focused on the association with susceptibility to GC, especially to EBVaGC.
Polymorphisms of the TLR2, 3, 4, and 9 genes were measured in 52 cases of EBVaGC and 157 cases of EBV-negative GC (EBVnGC). Ninety-four peripheral blood samples from healthy individuals were also examined.
For the TLR2 gene (196 to 174 del), there was no significant difference between the GC group and control group in genotype, but there was a significant difference in the del allele. As for the TLR3 gene (c. 1377C/T), there were significant differences between the GC group and the control group in both genotype and allelic frequency. No SNPs single nucleotide polymorphisms (SNPs) were found in the TLR4 gene at the sites Asp299Gly and Thr399Ile. As for TLR9 1486T/C (rs187084) and C2848T (rs352140), there was also no association between the GC group and control. In all of the indicators, there were no significant differences between EBVaGCs and EBVnGCs.
The TLR3 gene (c. 1377C/T) polymorphisms and the del allele of the TLR2 gene ( 196 to 174) were both associated with susceptibility to GC in Shangdong Province of Northern China. There was no interaction between EBV and TLR gene polymorphisms in EBVaGC.
背景/目的：已鉴定出Toll样受体（TLR）基因中的多种多态性，并发现其与多种恶性肿瘤的易感性相关，如胃癌（GC）、乳腺癌和前列腺癌。然而，在中国北方，关于TLR基因多态性与GC易感性，尤其是与爱泼斯坦-巴尔病毒相关胃癌（EBVaGC）的易感性了解甚少。我们重点研究了与GC易感性，尤其是与EBVaGC易感性的关联。
对52例EBVaGC患者和157例EBV阴性GC（EBVnGC）患者的TLR2、3、4和9基因多态性进行了检测。还检测了94份健康个体的外周血样本。
对于TLR2基因（196至174缺失），GC组与对照组在基因型上无显著差异，但在缺失等位基因上存在显著差异。对于TLR3基因（c.1377C/T），GC组与对照组在基因型和等位基因频率上均存在显著差异。在TLR4基因的Asp299Gly和Thr399Ile位点未发现单核苷酸多态性（SNP）。对于TLR9的1486T/C（rs187084）和C2848T（rs352140），GC组与对照组之间也无关联。在所有指标中，EBVaGC与EBVnGC之间无显著差异。
TLR3基因（c.1377C/T）多态性和TLR2基因（196至174）的缺失等位基因均与中国北方山东省GC的易感性相关。在EBVaGC中，EBV与TLR基因多态性之间不存在相互作用。
