杜仲炮制后产物的化学成分研究

[Investigation on chemical constituents of processed products of Eucommiae Cortex].

作者信息

Tao Yi, Sheng Chen, Li Wei-dong, Cai Bao-chang, Lu Tu-lin

出版信息

Zhongguo Zhong Yao Za Zhi. 2014 Nov;39(22):4352-5.

Abstract

According to the 2010 Chinese pharmacopeia, salt processed and charcoal processed Eucommiae Cortex were pre- pared. HPLC-DAD analysis of the content of the bark and leaf of Eucommiae Cortex showed that the bark of Eucommiae Cortex mainly contained lignans such as pinoresinol glucose and iridoid including genipin, geniposide, geniposidic acid, while the leaf of Eucommiae Cortex consisted of flavonoids such as quercetin and phenolic compound such as chlorogenic acid. The content of pinoresinol diglucoside in the bark of Eucommiae Cortex was about 18 times more than that in the leaf of Eucommiae Cortex. The content of pinoresinol diglucoside in salted and charcoal processed Eucommiae Cortex decreased approximately by 30% and 85%, respectively. The content of genipin, geniposide and geniposidic acid in the bark of Eucommiae Cortex was about 3 times, 23 times, 28 times more than that in the leaf of Eucommiae Cortex. The content of genipin, geniposide and geniposidic acid in salted Eucommiae Cortex were reduced by 25%, 40% and 40%, respectively. The content of genipin, geniposide and geniposidic acid in charcoal processed Eucommiae Cortex were reduced by 98%, 70%, 70%, respectively. The content of caffeic acid in bark of Eucommiae Cortex was about 3 times more than that in the leaf of Eucommiae Cortex. The content of caffeic acid was decreased by about 50% in the salted Eucommiae Cortex. While the content of caffeic acid in charcoal processed Eucommiae Cortex was decreased approximately 75%; the content of chlorogenic acid in bark of Eucommiae Cortex was about 1/6 of that in the leaf of Eucommiae Cortex. The content of chlorogenic acid in salted and charcoal processed Eucommiae Cortex decreased by 40% and 75%, respectively; the content of quercetin in bark of Eucommiae Cortex was only 1/40 of that in the leaf of Eucommiae Cortex. The content of quercetin in salted and charcoal processed Eucommiae Cortex were reduced by 60% and 50%, respectively.

摘要

根据2010年《中国药典》，制备了盐炙和炒炭的杜仲皮。对杜仲皮的树皮和树叶成分进行HPLC-DAD分析，结果表明，杜仲皮的树皮主要含有松脂醇葡萄糖等木脂素类成分，以及京尼平、京尼平苷、京尼平苷酸等环烯醚萜类成分；而杜仲皮的树叶主要含有槲皮素等黄酮类成分以及绿原酸等酚类化合物。杜仲皮树皮中松脂醇二葡萄糖苷的含量约为杜仲皮树叶的18倍。盐炙和炒炭杜仲皮中松脂醇二葡萄糖苷的含量分别下降了约30%和85%。杜仲皮树皮中京尼平、京尼平苷和京尼平苷酸的含量分别约为杜仲皮树叶的3倍、23倍、28倍。盐炙杜仲皮中京尼平、京尼平苷和京尼平苷酸的含量分别降低了25%、40%和40%。炒炭杜仲皮中京尼平、京尼平苷和京尼平苷酸的含量分别降低了98%、70%、70%。杜仲皮树皮中咖啡酸的含量约为杜仲皮树叶的3倍。盐炙杜仲皮中咖啡酸的含量下降了约50%。而炒炭杜仲皮中咖啡酸的含量下降了约75%；杜仲皮树皮中绿原酸的含量约为杜仲皮树叶的1/6。盐炙和炒炭杜仲皮中绿原酸的含量分别下降了40%和75%；杜仲皮树皮中槲皮素的含量仅为杜仲皮树叶的1/40。盐炙和炒炭杜仲皮中槲皮素的含量分别降低了60%和50%。